Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor

Joseph J. Yohn, Christopher Smith, Troy Stevens, Joseph G. Morelli, Libby R. Shurnas, Sandra J. Walchak, Talia A. Hoffman, Kim K. Kelley, Antonio Escobedo-Morse, Masashi Yanagisawa, Madeleine A. Kane, Martin R. Zamora

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7% decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.

Original languageEnglish (US)
Pages (from-to)454-458
Number of pages5
JournalBBA - Molecular Cell Research
Volume1224
Issue number3
DOIs
StatePublished - Dec 30 1994

Fingerprint

Endothelin B Receptors
Endothelin-1
Keratinocytes
Homeostasis
Endothelin A Receptors
Conditioned Culture Medium
Protein Isoforms
Calcium

Keywords

  • (Human)
  • Calcium
  • Endothelin-1
  • Keratinocyte
  • Receptor

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Yohn, J. J., Smith, C., Stevens, T., Morelli, J. G., Shurnas, L. R., Walchak, S. J., ... Zamora, M. R. (1994). Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor. BBA - Molecular Cell Research, 1224(3), 454-458. https://doi.org/10.1016/0167-4889(94)90282-8

Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor. / Yohn, Joseph J.; Smith, Christopher; Stevens, Troy; Morelli, Joseph G.; Shurnas, Libby R.; Walchak, Sandra J.; Hoffman, Talia A.; Kelley, Kim K.; Escobedo-Morse, Antonio; Yanagisawa, Masashi; Kane, Madeleine A.; Zamora, Martin R.

In: BBA - Molecular Cell Research, Vol. 1224, No. 3, 30.12.1994, p. 454-458.

Research output: Contribution to journalArticle

Yohn, JJ, Smith, C, Stevens, T, Morelli, JG, Shurnas, LR, Walchak, SJ, Hoffman, TA, Kelley, KK, Escobedo-Morse, A, Yanagisawa, M, Kane, MA & Zamora, MR 1994, 'Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor', BBA - Molecular Cell Research, vol. 1224, no. 3, pp. 454-458. https://doi.org/10.1016/0167-4889(94)90282-8
Yohn, Joseph J. ; Smith, Christopher ; Stevens, Troy ; Morelli, Joseph G. ; Shurnas, Libby R. ; Walchak, Sandra J. ; Hoffman, Talia A. ; Kelley, Kim K. ; Escobedo-Morse, Antonio ; Yanagisawa, Masashi ; Kane, Madeleine A. ; Zamora, Martin R. / Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor. In: BBA - Molecular Cell Research. 1994 ; Vol. 1224, No. 3. pp. 454-458.
@article{46d2c9d684fc40ed9a1907d7af59a582,
title = "Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor",
abstract = "We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7{\%} decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.",
keywords = "(Human), Calcium, Endothelin-1, Keratinocyte, Receptor",
author = "Yohn, {Joseph J.} and Christopher Smith and Troy Stevens and Morelli, {Joseph G.} and Shurnas, {Libby R.} and Walchak, {Sandra J.} and Hoffman, {Talia A.} and Kelley, {Kim K.} and Antonio Escobedo-Morse and Masashi Yanagisawa and Kane, {Madeleine A.} and Zamora, {Martin R.}",
year = "1994",
month = "12",
day = "30",
doi = "10.1016/0167-4889(94)90282-8",
language = "English (US)",
volume = "1224",
pages = "454--458",
journal = "Biochimica et Biophysica Acta - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor

AU - Yohn, Joseph J.

AU - Smith, Christopher

AU - Stevens, Troy

AU - Morelli, Joseph G.

AU - Shurnas, Libby R.

AU - Walchak, Sandra J.

AU - Hoffman, Talia A.

AU - Kelley, Kim K.

AU - Escobedo-Morse, Antonio

AU - Yanagisawa, Masashi

AU - Kane, Madeleine A.

AU - Zamora, Martin R.

PY - 1994/12/30

Y1 - 1994/12/30

N2 - We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7% decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.

AB - We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7% decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.

KW - (Human)

KW - Calcium

KW - Endothelin-1

KW - Keratinocyte

KW - Receptor

UR - http://www.scopus.com/inward/record.url?scp=0028574308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028574308&partnerID=8YFLogxK

U2 - 10.1016/0167-4889(94)90282-8

DO - 10.1016/0167-4889(94)90282-8

M3 - Article

C2 - 7803503

AN - SCOPUS:0028574308

VL - 1224

SP - 454

EP - 458

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 3

ER -