Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor

Joseph J. Yohn, Christopher Smith, Troy Stevens, Joseph G. Morelli, Libby R. Shurnas, Sandra J. Walchak, Talia A. Hoffman, Kim K. Kelley, Antonio Escobedo-Morse, Masashi Yanagisawa, Madeleine A. Kane, Martin R. Zamora

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7% decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.

Original languageEnglish (US)
Pages (from-to)454-458
Number of pages5
JournalBBA - Molecular Cell Research
Volume1224
Issue number3
DOIs
StatePublished - Dec 30 1994

Keywords

  • (Human)
  • Calcium
  • Endothelin-1
  • Keratinocyte
  • Receptor

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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