Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini- and Conventional STR Kits

Chang Seok Oh, Sang Jun Lee, Jun Bum Park, Soong Deok Lee, Seung Bum Seo, Hye Yeon Kim, Jaehyup Kim, Yi Suk Kim, Dong Hoon Shin

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFℓSTR ® MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n=8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFℓSTR ® MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger-sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large-sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples.

Original languageEnglish (US)
Pages (from-to)820-825
Number of pages6
JournalJournal of Forensic Sciences
Volume57
Issue number3
DOIs
StatePublished - May 1 2012
Externally publishedYes

Keywords

  • AmpFℓSTR Identifiler™ kit
  • AmpFℓSTR MiniFiler™ kit
  • Ancient DNA
  • Autosomal short tandem repeat genotyping
  • Forensic science
  • Korea
  • Medieval human sample

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Genetics

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  • Cite this

    Oh, C. S., Lee, S. J., Park, J. B., Lee, S. D., Seo, S. B., Kim, H. Y., Kim, J., Kim, Y. S., & Shin, D. H. (2012). Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini- and Conventional STR Kits. Journal of Forensic Sciences, 57(3), 820-825. https://doi.org/10.1111/j.1556-4029.2011.02044.x