AUUUA-specific mRNA binding proteins in astrocytes

David B. Jacobs; Arthur M. Mandelin; Tony Giordano; Iris Xue; James S. Malter; Leelabai D. Singh; Ann K. Snyder; Sant P. Singh

doi:10.1016/0024-3205(96)00202-0

AUUUA-specific mRNA binding proteins in astrocytes

David B. Jacobs, Arthur M. Mandelin, Tony Giordano, Iris Xue, James S. Malter, Leelabai D. Singh, Ann K. Snyder, Sant P. Singh

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Binding of ribonucleoproteins to specific regions of mRNA can alter mRNA stability. This level of posttranscriptional regulation has been shown to play a major role in gene expression of eukaryotic cells. This process involves the binding of ribonucleoproteins to specific region(s) of unstable, rapidly degrading mRNAs such as those found in various cytokines, lymphokines, and oncogenes, thereby increasing the mRNA's stability. In many instances the instability of the mRNA has been mapped to an AU-rich motif in the 3' untranslated region. We transcribed RNA molecules containing four reiterations of an AUUUA motif, and demonstrated with RNA- band shift experiments that the AUUUA motif complexes with phosphorylated AUUUA-specific 43-47 kDa mRNA binding protein(s) found in the cytosol of both rat brain and cultured rat astrocytes.

Original language	English (US)
Pages (from-to)	2083-2089
Number of pages	7
Journal	Life Sciences
Volume	58
Issue number	23
DOIs	https://doi.org/10.1016/0024-3205(96)00202-0
State	Published - May 3 1996

Keywords

Astrocytes
Phosphorylation
mRNA binding proteins

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(96)00202-0

Cite this

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title = "AUUUA-specific mRNA binding proteins in astrocytes",

abstract = "Binding of ribonucleoproteins to specific regions of mRNA can alter mRNA stability. This level of posttranscriptional regulation has been shown to play a major role in gene expression of eukaryotic cells. This process involves the binding of ribonucleoproteins to specific region(s) of unstable, rapidly degrading mRNAs such as those found in various cytokines, lymphokines, and oncogenes, thereby increasing the mRNA's stability. In many instances the instability of the mRNA has been mapped to an AU-rich motif in the 3' untranslated region. We transcribed RNA molecules containing four reiterations of an AUUUA motif, and demonstrated with RNA- band shift experiments that the AUUUA motif complexes with phosphorylated AUUUA-specific 43-47 kDa mRNA binding protein(s) found in the cytosol of both rat brain and cultured rat astrocytes.",

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T1 - AUUUA-specific mRNA binding proteins in astrocytes

AU - Jacobs, David B.

AU - Mandelin, Arthur M.

AU - Giordano, Tony

AU - Xue, Iris

AU - Malter, James S.

AU - Singh, Leelabai D.

AU - Snyder, Ann K.

AU - Singh, Sant P.

PY - 1996/5/3

Y1 - 1996/5/3

N2 - Binding of ribonucleoproteins to specific regions of mRNA can alter mRNA stability. This level of posttranscriptional regulation has been shown to play a major role in gene expression of eukaryotic cells. This process involves the binding of ribonucleoproteins to specific region(s) of unstable, rapidly degrading mRNAs such as those found in various cytokines, lymphokines, and oncogenes, thereby increasing the mRNA's stability. In many instances the instability of the mRNA has been mapped to an AU-rich motif in the 3' untranslated region. We transcribed RNA molecules containing four reiterations of an AUUUA motif, and demonstrated with RNA- band shift experiments that the AUUUA motif complexes with phosphorylated AUUUA-specific 43-47 kDa mRNA binding protein(s) found in the cytosol of both rat brain and cultured rat astrocytes.

AB - Binding of ribonucleoproteins to specific regions of mRNA can alter mRNA stability. This level of posttranscriptional regulation has been shown to play a major role in gene expression of eukaryotic cells. This process involves the binding of ribonucleoproteins to specific region(s) of unstable, rapidly degrading mRNAs such as those found in various cytokines, lymphokines, and oncogenes, thereby increasing the mRNA's stability. In many instances the instability of the mRNA has been mapped to an AU-rich motif in the 3' untranslated region. We transcribed RNA molecules containing four reiterations of an AUUUA motif, and demonstrated with RNA- band shift experiments that the AUUUA motif complexes with phosphorylated AUUUA-specific 43-47 kDa mRNA binding protein(s) found in the cytosol of both rat brain and cultured rat astrocytes.

KW - Astrocytes

KW - Phosphorylation

KW - mRNA binding proteins

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ER -

AUUUA-specific mRNA binding proteins in astrocytes

Abstract

Keywords

ASJC Scopus subject areas

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