Avian and murine lr8b and human apolipoprotein E receptor 2: Differentially spliced products from corresponding genes

Christian Brandes, Sabine Novak, Walter Stockinger, Joachim Herz, Wolfgang J. Schneider, Johannes Nimpf

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Apolipoprotein E-mediated lipid metabolism in the central nervous system plays an important role in cholesterol and phospholipid homeostasis of this organ, which is separated from the circulation by the blood-brain barrier. Moreover, in late-onset familial Alzheimer disease the frequency of the apolipoprotein E4 allele is significantly increased and the apoprotein is localized to extracellular plaques, one of the histological hallmarks of this disease. Recently, two distinct novel members of the low-density lipoprotein (LDL) receptor family, with the potential to bind apolipoprotein E and preferentially expressed in brain, have been characterized from human (D. Kim et al., 1996, J. Biol. Chem 271: 8373-8380) and chicken and mouse (S. Novak, et al., 1996, J. Biol. Chem. 271: 11732-11736). The human receptor, termed 'apolipoprotein E receptor 2,' is a seven ligand-binding repeat receptor harboring a unique insertion in the cytoplasmic domain of the protein. The novel receptor characterized in chicken and mouse was found to have eight binding repeats without such a cytoplasmic insertion. Despite the overall identity of more than 73%, based upon their structural differences (seven versus eight ligand-binding repeats) these receptors have been considered independent entities. However, here we demonstrate that both receptors in fact are encoded by corresponding genes and that differential splicing gives rise to structurally and possibly functionally distinct variants of this brain-specific member of the LDL receptor family.

Original languageEnglish (US)
Pages (from-to)185-191
Number of pages7
JournalGenomics
Volume42
Issue number2
DOIs
StatePublished - Jun 1 1997

Fingerprint

LDL Receptors
Apolipoproteins E
Chickens
Ligands
Apolipoprotein E4
Apoproteins
Brain
Blood-Brain Barrier
Lipid Metabolism
Genes
Phospholipids
Homeostasis
Central Nervous System
Alleles
Cholesterol
low density lipoprotein receptor-related protein 8
Alzheimer disease type 2
Protein Domains

ASJC Scopus subject areas

  • Genetics

Cite this

Avian and murine lr8b and human apolipoprotein E receptor 2 : Differentially spliced products from corresponding genes. / Brandes, Christian; Novak, Sabine; Stockinger, Walter; Herz, Joachim; Schneider, Wolfgang J.; Nimpf, Johannes.

In: Genomics, Vol. 42, No. 2, 01.06.1997, p. 185-191.

Research output: Contribution to journalArticle

Brandes, Christian ; Novak, Sabine ; Stockinger, Walter ; Herz, Joachim ; Schneider, Wolfgang J. ; Nimpf, Johannes. / Avian and murine lr8b and human apolipoprotein E receptor 2 : Differentially spliced products from corresponding genes. In: Genomics. 1997 ; Vol. 42, No. 2. pp. 185-191.
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