B-1a Cell Development in Splenectomized Neonatal Mice

Gabriel K. Pedersen, Xiaohong Li, Sharesta Khoenkhoen, Monika Ádori, Bruce Beutler, Gunilla B. Karlsson Hedestam

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2 Scopus citations

Abstract

B-1a cells are mainly generated from fetal liver progenitor cells, peri- and neonatally. The developmental steps and anatomical sites required for these cells to become mature B-1a cells remain elusive. We recently described a phenotypically distinct transitional B cell subset in the spleen of neonatal mice that generated B-1a cells when adoptively transferred. This, in combination with findings demonstrating that B-1a cells are lacking in congenitally asplenic mice, led us to hypothesize that the neonatal spleen is required for B-1a cell development. In accordance with previous reports, we found that B-1a cell numbers were reduced in adult mice that had undergone splenectomy compared to after sham surgery. In contrast, neonatal splenectomy led to peritoneal B-1a cell frequencies comparable to those observed in sham-operated mice until 6 weeks after surgery, suggesting that an intact spleen is required for B-1a cell maintenance rather than development. To study the role of the prenatal spleen in generating B-1a cells, we transferred fetal liver cells from pre-splenic embryos [embryonic age 11 (E11) days] into splenectomized recipient mice. B-1a cells were generated in the absence of the spleen, albeit at slightly reduced frequencies, and populated the peritoneal cavity and bone marrow. Lower bone marrow B-1a cell frequencies were also observed both after neonatal and adult splenectomy. These results demonstrated that B-1a cells could be generated in the complete absence of an intact spleen, but that asplenia led to a decline in these cells, suggesting a role of the spleen for maintaining the B-1a compartment.

Original languageEnglish (US)
Article number1738
Pages (from-to)1738
Number of pages1
JournalFrontiers in immunology
Volume9
DOIs
StatePublished - 2018

Keywords

  • B cell progenitors
  • B-1 cells
  • fetal liver
  • splenectomy
  • transitional B cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Pedersen, G. K., Li, X., Khoenkhoen, S., Ádori, M., Beutler, B., & Karlsson Hedestam, G. B. (2018). B-1a Cell Development in Splenectomized Neonatal Mice. Frontiers in immunology, 9, 1738. [1738]. https://doi.org/10.3389/fimmu.2018.01738