B cell tolerance. I. Analysis of hapten-specific unresponsiveness induced in vitro in adult and neonatal murine spleen cell populations

J. C. Cambier, J. W. Uhr, J. R. Kettman, E. S. Vitetta

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8 Citations (Scopus)

Abstract

The cellular mechanisms and tolerogen dose requirements of hapten-specific unresponsiveness induced in vitro by using 2,4,6-trinitrophenyl human γ-globulin (TNP17HgG) were analyzed in adult and neonatal murine splenocytes. Tolerance induction in both cell populations was found to be independent of non-B cell effects including BAθ-positive cells, Ly 2.2-positive cells, adding or reducing the number of macrophages, and large excesses of HgG. The tolerance induced was specific and not 'infectious', further excluding a role for suppressor T cells. Neonatal splenic B cells were rendered tolerant by doses of TNP17HgG 1000-fold less than those required to produce similar tolerance in splenic B cells from adults. These findings support the concept of functional clonal abortion as a mechanism for producing tolerance to self antigens.

Original languageEnglish (US)
Pages (from-to)2054-2059
Number of pages6
JournalJournal of Immunology
Volume119
Issue number6
StatePublished - 1977

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Haptens
B-Lymphocytes
Spleen
Population
Clonal Deletion
Globulins
Autoantigens
Macrophages
T-Lymphocytes
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

B cell tolerance. I. Analysis of hapten-specific unresponsiveness induced in vitro in adult and neonatal murine spleen cell populations. / Cambier, J. C.; Uhr, J. W.; Kettman, J. R.; Vitetta, E. S.

In: Journal of Immunology, Vol. 119, No. 6, 1977, p. 2054-2059.

Research output: Contribution to journalArticle

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