TY - JOUR
T1 - B lymphocytes in human subcutaneous adipose crown-like structures
AU - McDonnell, Marie E.
AU - Ganley-Leal, Lisa M.
AU - Mehta, Ankeeta
AU - Bigornia, Sherman J.
AU - Mott, Melanie
AU - Rehman, Qasim
AU - Farb, Melissa G.
AU - Hess, Donald T.
AU - Joseph, Lija
AU - Gokce, Noyan
AU - Apovian, Caroline M.
PY - 2012/7
Y1 - 2012/7
N2 - Accumulation of macrophages and T cells within crown-like structures (CLS) in subcutaneous adipose tissue predicts disease severity in obesity-related insulin resistance (OIR). Although rodent data suggest the B cell is an important feature of these lesions, B cells have not been described within the human CLS. In order to identify B cells in the human subcutaneous CLS (sCLS) in obese subjects and determine whether the presence of B cells predict insulin resistance, we examined archived samples of subcutaneous and omental fat from 32 obese men and women and related findings to clinical parameters. Using immunohistochemistry, we identified B (CD19 ) and T cells (CD3 ) within the sCLS and perivascular space. The presence and density of B cells (B cells per high-power field (pHPF), T cells pHPF, and B cell:T cell (B:T) ratio) were compared with measures of insulin resistance (homeostasis model assessment (HOMA)) and other variables. In 16 of 32 subjects (50%) CD19 B cells were localized within sCLS and were relatively more numerous than T cells. HOMA was not different between subjects with CD19 vs. CD19 sCLS (5.5 vs. 5.3, P = 0.88). After controlling for diabetes and glycemia (hemoglobin A 1c (HbA 1c)), the B:T ratio correlated with current metformin treatment (r = 0.89, P = 0.001). These results indicate that in human OIR, B cells are an integral component of organized inflammation in subcutaneous fat, and defining their role will lead to a better understanding of OIR pathogenesis and potentially impact treatment.
AB - Accumulation of macrophages and T cells within crown-like structures (CLS) in subcutaneous adipose tissue predicts disease severity in obesity-related insulin resistance (OIR). Although rodent data suggest the B cell is an important feature of these lesions, B cells have not been described within the human CLS. In order to identify B cells in the human subcutaneous CLS (sCLS) in obese subjects and determine whether the presence of B cells predict insulin resistance, we examined archived samples of subcutaneous and omental fat from 32 obese men and women and related findings to clinical parameters. Using immunohistochemistry, we identified B (CD19 ) and T cells (CD3 ) within the sCLS and perivascular space. The presence and density of B cells (B cells per high-power field (pHPF), T cells pHPF, and B cell:T cell (B:T) ratio) were compared with measures of insulin resistance (homeostasis model assessment (HOMA)) and other variables. In 16 of 32 subjects (50%) CD19 B cells were localized within sCLS and were relatively more numerous than T cells. HOMA was not different between subjects with CD19 vs. CD19 sCLS (5.5 vs. 5.3, P = 0.88). After controlling for diabetes and glycemia (hemoglobin A 1c (HbA 1c)), the B:T ratio correlated with current metformin treatment (r = 0.89, P = 0.001). These results indicate that in human OIR, B cells are an integral component of organized inflammation in subcutaneous fat, and defining their role will lead to a better understanding of OIR pathogenesis and potentially impact treatment.
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U2 - 10.1038/oby.2012.54
DO - 10.1038/oby.2012.54
M3 - Article
C2 - 22395812
AN - SCOPUS:84862888806
SN - 1930-7381
VL - 20
SP - 1372
EP - 1378
JO - Obesity
JF - Obesity
IS - 7
ER -