B lymphocytes induce the formation of follicular dendritic cell clusters in a lymphotoxin α-dependent fashion

Yang Xin Fu, Guangming Huang, Yang Wang, David D. Chaplin

Research output: Contribution to journalArticle

232 Citations (Scopus)

Abstract

Lymphotoxin (LT)α is expressed by activated T cells, especially CD4+ T helper type 1 cells, and by activated B and natural killer cells, but the functions of this molecule in vivo are incompletely defined. We have previously shown that follicular dendritic cell (FDC) clusters and germinal centers (GCs) are absent from the peripheral lymphoid tissues of LTα- deficient (LTα(-/-) mice. LTα(-/-) mice produce high levels of antigen- specific immunoglobulin (Ig)M, but very low levels of IgG after immunization with sheep red blood cells. We show here that LTα-expressing B cells are essential for the recovery of primary, secondary, and memory humoral immune responses in LTα(-/-) mice. It is not necessary for T cells to express LTα to support these immune functions. Importantly, LTα-expressing B cells alone are essential and sufficient for the formation of FDC clusters. Once these clusters are formed by LTα-expressing B cells, then LTα-deficient T cells can interact with B cells to generate GCs and productive class-switched antibody responses. Thus, B cells themselves provide an essential signal that induces and maintains the lymphoid microenvironment essential for GC formation and class-switched Ig responses.

Original languageEnglish (US)
Pages (from-to)1009-1018
Number of pages10
JournalJournal of Experimental Medicine
Volume187
Issue number7
DOIs
StatePublished - Apr 6 1998

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Follicular Dendritic Cells
Lymphotoxin-alpha
B-Lymphocytes
Germinal Center
T-Lymphocytes
Th1 Cells
Immunoglobulin Isotypes
Lymphoid Tissue
Humoral Immunity
Natural Killer Cells
Antibody Formation
Immunoglobulin M
Immunization
Sheep
Immunoglobulin G
Erythrocytes

ASJC Scopus subject areas

  • Immunology

Cite this

B lymphocytes induce the formation of follicular dendritic cell clusters in a lymphotoxin α-dependent fashion. / Fu, Yang Xin; Huang, Guangming; Wang, Yang; Chaplin, David D.

In: Journal of Experimental Medicine, Vol. 187, No. 7, 06.04.1998, p. 1009-1018.

Research output: Contribution to journalArticle

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