B55α PP2A holoenzymes modulate the phosphorylation status of the retinoblastoma-related protein p107 and its activation

Girish Jayadeva, Alison Kurimchak, Judit Garriga, Elena Sotillo, Anthony J. Davis, Dale S. Haines, Marc Mumby, Xavier Graña

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Pocket proteins negatively regulate transcription of E2F-dependent genes and progression through the G0/G1 transition and the cell cycle restriction point in G1. Pocket protein repressor activities are inactivated via phosphorylation at multiple Pro-directed Ser/Thr sites by the coordinated action of G1 and G1/S cyclin-dependent kinases. These phosphorylations are reversed by the action of two families of Ser/Thr phosphatases: PP1, which has been implicated in abrupt dephosphorylation of retinoblastoma protein (pRB) in mitosis, and PP2A, which plays a role in an equilibrium that counteracts cyclin-dependent kinase (CDK) action throughout the cell cycle. However, the identity of the trimeric PP2A holoenzyme(s) functioning in this process is unknown. Here we report the identification of a PP2A trimeric holoenzyme containing B55α, which plays a major role in restricting the phosphorylation state of p107 and inducing its activation in human cells. Our data also suggest targeted selectivity in the interaction of pocket proteins with distinct PP2A holoenzymes, which is likely necessary for simultaneous pocket protein activation.

Original languageEnglish (US)
Pages (from-to)29863-29873
Number of pages11
JournalJournal of Biological Chemistry
Volume285
Issue number39
DOIs
StatePublished - Sep 24 2010

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Retinoblastoma Protein
Holoenzymes
Phosphorylation
Cyclin-Dependent Kinases
Chemical activation
Cells
Repressor Proteins
Proteins
Transcription
Cell Cycle Checkpoints
Phosphoric Monoester Hydrolases
Mitosis
Cell Cycle
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

B55α PP2A holoenzymes modulate the phosphorylation status of the retinoblastoma-related protein p107 and its activation. / Jayadeva, Girish; Kurimchak, Alison; Garriga, Judit; Sotillo, Elena; Davis, Anthony J.; Haines, Dale S.; Mumby, Marc; Graña, Xavier.

In: Journal of Biological Chemistry, Vol. 285, No. 39, 24.09.2010, p. 29863-29873.

Research output: Contribution to journalArticle

Jayadeva, Girish ; Kurimchak, Alison ; Garriga, Judit ; Sotillo, Elena ; Davis, Anthony J. ; Haines, Dale S. ; Mumby, Marc ; Graña, Xavier. / B55α PP2A holoenzymes modulate the phosphorylation status of the retinoblastoma-related protein p107 and its activation. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 39. pp. 29863-29873.
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