Bacterial exposure induces and activates matrilysin in mucosal epithelial cells

Yolanda S. López-Boado, Carole L. Wilson, Lora V. Hooper, Jeffrey I. Gordon, Scott J. Hultgren, William C. Parks

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Matrilysin, a matrix metalloproteinase, is expressed and secreted lumenally by intact mucosal and glandular epithelia throughout the body, suggesting that its regulation and function are shared among tissues. Because matrilysin is produced in Paneth cells of the murine small intestine, where it participates in innate host defense by activation of prodefensins, we speculated that its expression would be influenced by bacterial exposure. Indeed, acute infection (10-90 min) of human colon, bladder, and lung carcinoma cells, primary human tracheal epithelial cells, and human tracheal explants with type 1-piliated Escherichia coli mediated a marked (25-50-fold) and sustained (>24 h) induction of matrilysin production. In addition, bacterial infection resulted in activation of the zymogen form of the enzyme, which was selectively released at the apical surface. Induction of matrilysin was mediated by a soluble, non-LPS bacterial factor and correlated with the release of defensin-like bacteriocidal activity. Bacteria did not induce matrilysin in other cell types, and expression of other metalloproteinases by epithelial cells was not affected by bacteria. Matrilysin was not detected in germ-free mice, but the enzyme was induced after colonization with Bacteroides thetaiotaomicron. These findings indicate that bacterial exposure is a potent and physiologically relevant signal regulating matrilysin expression in epithelial cells.

Original languageEnglish (US)
Pages (from-to)1305-1315
Number of pages11
JournalJournal of Cell Biology
Volume148
Issue number6
DOIs
StatePublished - Mar 20 2000

Fingerprint

Matrix Metalloproteinase 7
Epithelial Cells
Paneth Cells
Bacteria
Defensins
Enzyme Precursors
Metalloproteases
Enzymes
Matrix Metalloproteinases
Bacterial Infections
Small Intestine
Colon
Urinary Bladder
Epithelium
Escherichia coli
Carcinoma
Lung
Infection

Keywords

  • Adhesin
  • Bacteria
  • Defensin
  • Host defense
  • Metalloproteinase

ASJC Scopus subject areas

  • Cell Biology

Cite this

López-Boado, Y. S., Wilson, C. L., Hooper, L. V., Gordon, J. I., Hultgren, S. J., & Parks, W. C. (2000). Bacterial exposure induces and activates matrilysin in mucosal epithelial cells. Journal of Cell Biology, 148(6), 1305-1315. https://doi.org/10.1083/jcb.148.6.1305

Bacterial exposure induces and activates matrilysin in mucosal epithelial cells. / López-Boado, Yolanda S.; Wilson, Carole L.; Hooper, Lora V.; Gordon, Jeffrey I.; Hultgren, Scott J.; Parks, William C.

In: Journal of Cell Biology, Vol. 148, No. 6, 20.03.2000, p. 1305-1315.

Research output: Contribution to journalArticle

López-Boado, YS, Wilson, CL, Hooper, LV, Gordon, JI, Hultgren, SJ & Parks, WC 2000, 'Bacterial exposure induces and activates matrilysin in mucosal epithelial cells', Journal of Cell Biology, vol. 148, no. 6, pp. 1305-1315. https://doi.org/10.1083/jcb.148.6.1305
López-Boado, Yolanda S. ; Wilson, Carole L. ; Hooper, Lora V. ; Gordon, Jeffrey I. ; Hultgren, Scott J. ; Parks, William C. / Bacterial exposure induces and activates matrilysin in mucosal epithelial cells. In: Journal of Cell Biology. 2000 ; Vol. 148, No. 6. pp. 1305-1315.
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