Bacterial lipopolysaccharide binding enhances virion stability and promotes environmental fitness of an enteric virus

Christopher M. Robinson, Palmy R. Jesudhasan, Julie K. Pfeiffer

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Summary Enteric viruses, including poliovirus and reovirus, encounter a vast microbial community in the mammalian gastrointestinal tract, which has been shown to promote virus replication and pathogenesis. Investigating the underlying mechanisms, we find that poliovirus binds bacterial surface polysaccharides, which enhances virion stability and cell attachment by increasing binding to the viral receptor. Additionally, we identified a poliovirus mutant, VP1-T99K, with reduced lipopolysaccharide (LPS) binding. Although T99K and WT poliovirus cell attachment, replication, and pathogenesis in mice are equivalent, VP1-T99K poliovirus was unstable in feces following peroral inoculation of mice. Consequently, the ratio of mutant virus in feces is reduced following additional cycles of infection in mice. Thus, the mutant virus incurs a fitness cost when environmental stability is a factor. These data suggest that poliovirus binds bacterial surface polysaccharides, enhancing cell attachment and environmental stability, potentially promoting transmission to a new host.

Original languageEnglish (US)
Pages (from-to)36-46
Number of pages11
JournalCell Host and Microbe
Volume15
Issue number1
DOIs
StatePublished - Jan 15 2014

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Poliovirus
Enterovirus
Virion
Lipopolysaccharides
Bacterial Polysaccharides
Feces
Viruses
Virus Replication
Gastrointestinal Tract
Costs and Cost Analysis
Infection

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Cancer Research
  • Molecular Biology

Cite this

Bacterial lipopolysaccharide binding enhances virion stability and promotes environmental fitness of an enteric virus. / Robinson, Christopher M.; Jesudhasan, Palmy R.; Pfeiffer, Julie K.

In: Cell Host and Microbe, Vol. 15, No. 1, 15.01.2014, p. 36-46.

Research output: Contribution to journalArticle

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