Bam and Bgcn antagonize Nanos-dependent germ-line stem cell maintenance

Yun Li, Nicole T. Minor, Joseph K. Park, Dennis M. McKearin, Jean Z. Maines

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

The balance between germ-line stem cell (GSC) self-renewal and differentiation in Drosophila ovaries is mediated by the antagonistic relationship between the Nanos (Nos)-Pumilio translational repressor complex, which promotes GSC self-renewal, and expression of Bam, a key differentiation factor. Here, we find that Bam and Nos proteins are expressed in reciprocal patterns in young germ cells. Repression of Nos in Bam-expressing cells depends on sequences in the nos 3′-UTR, suggesting that Nos is regulated by translational repression. Ectopic Bam causes differentiation of GSCs, and this activity depends on the endogenous nos 3′-UTR sequence. Previous evidence showed that Bgcn is an obligate factor for the ability of Bam to drive differentiation, and we now report that Bam forms a complex with Bgcn, a protein related to the RNA-interacting DExH-box polypeptides. Together, these observations suggest that Bam-Bgcn act together to antagonize Nos expression; thus, derepressing cystoblast-promoting factors. These findings emphasize the importance of translational repression in balancing stem cell self-renewal and differentiation.

Original languageEnglish (US)
Pages (from-to)9304-9309
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number23
DOIs
StatePublished - Jun 9 2009

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Germ Cells
Stem Cells
Maintenance
3' Untranslated Regions
Drosophila
Ovary
Proteins
RNA
Peptides
Cell Self Renewal

Keywords

  • Drosophila
  • Germ cell
  • Oogenesis

ASJC Scopus subject areas

  • General

Cite this

Bam and Bgcn antagonize Nanos-dependent germ-line stem cell maintenance. / Li, Yun; Minor, Nicole T.; Park, Joseph K.; McKearin, Dennis M.; Maines, Jean Z.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 23, 09.06.2009, p. 9304-9309.

Research output: Contribution to journalArticle

Li, Yun ; Minor, Nicole T. ; Park, Joseph K. ; McKearin, Dennis M. ; Maines, Jean Z. / Bam and Bgcn antagonize Nanos-dependent germ-line stem cell maintenance. In: Proceedings of the National Academy of Sciences of the United States of America. 2009 ; Vol. 106, No. 23. pp. 9304-9309.
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