Basement membrane and human epidermal differentiation in vitro

Research output: Contribution to journalArticle

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Abstract

To test the role of basement membrane in differentiation of human epidermis reconstituted on human dermis, we prepared dermis with and without basement membrane and cultured epidermal cells on these two dermal substrata. The absence of basement membrane components was confirmed by immunofluorescence staining for laminin and type IV collagen and by electron microscopy. A high degree of differentiation of reconstituted epidermis did not require basement membrane as shown by the development of basal, spinous, and granular cell layers, and synthesis of 58 and 65-67 kDa keratins when epidermis was attached directly to dermis. On the other hand, we found that the basement membrane regulated the adhesive interaction between the epidermis and dermis. On dermis with basement membrane, attached epidermal cells formed hemidesmosomes and mechanically stable bonding. In the absence of basement membrane, the epidermal cells did not form hemidesmosomes, and bonding between the epidermis and dermis was unstable. Moreover, dermis from which the basement membrane was removed was reorganized by the epidermal cell layer.

Original languageEnglish (US)
Pages (from-to)372-378
Number of pages7
JournalJournal of Investigative Dermatology
Volume93
Issue number3
StatePublished - Sep 1989

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Basement Membrane
Dermis
Epidermis
Hemidesmosomes
Cells
Collagen Type IV
In Vitro Techniques
Laminin
Keratins
Adhesives
Electron microscopy
Fluorescent Antibody Technique
Cultured Cells
Electron Microscopy
Staining and Labeling
Skin

ASJC Scopus subject areas

  • Dermatology

Cite this

Basement membrane and human epidermal differentiation in vitro. / Guo, Ming; Grinnell, Frederick.

In: Journal of Investigative Dermatology, Vol. 93, No. 3, 09.1989, p. 372-378.

Research output: Contribution to journalArticle

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