Bell-shaped calcium-response curves of Ins(1,4,S)P3- and calcium-gated channels from endoplasmic reticulum of cerebellum

Ilya Bezprozvanny, James Watras, Barbara E. Ehrlich

Research output: Contribution to journalArticle

1318 Citations (Scopus)

Abstract

RELEASE of calcium from intracellular stores occurs by two pathways, an inositol 1,4,5-trisphosphate (InsP3)-gated channel1-3 and a calcium-gated channel (ryanodine receptor)4-6. Using specific antibodies, both receptors were found in Purkinje cells of cerebellum7,8. We have now compared the functional properties of the channels corresponding to the two receptors by incorporating endoplasmic reticulum vesicles from canine cerebellum into planar bilayers. InsP3-gated channels were observed most frequently. Another channel type was activated by adenine nucleotides or caffeine, inhibited by ruthenium red, and modified by ryanodine, characteristics of the ryanodine receptor/channel6. The open probability of both channel types displayed a bell-shaped curve for dependence on calcium. For the InsP3-gated channel, the maximum probability of opening occurred at 0.2 μM free calcium, with sharp decreases on either side of the maximum. Maximum activity for the ryanodine receptor/channel was maintained between 1 and 100 μM calcium. Thus, within the physiological range of cytoplasmic calcium, the InsP3-gated channel itself allows positive feed-back and then negative feedback for calcium release, whereas the ryanodine receptor/channel behaves solely as a calcium-activated channel. The existence in the same cell of two channels with different responses to calcium and different ligand sensitivities provides a basis for complex patterns of intracellular calcium regulation.

Original languageEnglish (US)
Pages (from-to)751-754
Number of pages4
JournalNature
Volume351
Issue number6329
StatePublished - Jun 27 1991

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Calcium Channels
Endoplasmic Reticulum
Cerebellum
Ryanodine Receptor Calcium Release Channel
Calcium
Ruthenium Red
Ryanodine
Inositol 1,4,5-Trisphosphate
Adenine Nucleotides
Purkinje Cells
Caffeine
Canidae
Ligands
Antibodies

ASJC Scopus subject areas

  • General

Cite this

Bell-shaped calcium-response curves of Ins(1,4,S)P3- and calcium-gated channels from endoplasmic reticulum of cerebellum. / Bezprozvanny, Ilya; Watras, James; Ehrlich, Barbara E.

In: Nature, Vol. 351, No. 6329, 27.06.1991, p. 751-754.

Research output: Contribution to journalArticle

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AB - RELEASE of calcium from intracellular stores occurs by two pathways, an inositol 1,4,5-trisphosphate (InsP3)-gated channel1-3 and a calcium-gated channel (ryanodine receptor)4-6. Using specific antibodies, both receptors were found in Purkinje cells of cerebellum7,8. We have now compared the functional properties of the channels corresponding to the two receptors by incorporating endoplasmic reticulum vesicles from canine cerebellum into planar bilayers. InsP3-gated channels were observed most frequently. Another channel type was activated by adenine nucleotides or caffeine, inhibited by ruthenium red, and modified by ryanodine, characteristics of the ryanodine receptor/channel6. The open probability of both channel types displayed a bell-shaped curve for dependence on calcium. For the InsP3-gated channel, the maximum probability of opening occurred at 0.2 μM free calcium, with sharp decreases on either side of the maximum. Maximum activity for the ryanodine receptor/channel was maintained between 1 and 100 μM calcium. Thus, within the physiological range of cytoplasmic calcium, the InsP3-gated channel itself allows positive feed-back and then negative feedback for calcium release, whereas the ryanodine receptor/channel behaves solely as a calcium-activated channel. The existence in the same cell of two channels with different responses to calcium and different ligand sensitivities provides a basis for complex patterns of intracellular calcium regulation.

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