Beta-amyloid precursor protein staining in nonhomicidal pediatric medicolegal autopsies

R. Ross Reichard, Charles L. White, Christa L. Hladik, David Dolinak

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Immunohistochemical staining for beta-amyloid precursor protein (βAPP) has been validated as a marker for axonal injury in adults surviving ≥2 hours after white matter damage. The significance of βAPP staining in pediatric brains and spinal cords is not as well established. We evaluated the white matter immunoreactivity for βAPP from a variety of pediatric medicolegal autopsies: natural disease (non-Sudden Infant Death Syndrome [SIDS]), SIDS, motor vehicle accidents, drowning, near-drowning, overlay, carbon monoxide toxicity, miscellaneous trauma, and mechanical asphyxia. The cases of carbon monoxide toxicity, motor vehicle accidents (death at scene), drowning (with resuscitation), and a natural (non-SIDS) death had no significant white matter staining. The traumatic deaths with a significant survival interval, a variety of natural deaths, the near-drowning case. and surprisingly, all SIDS had detectable βAPP white matter immunostaining. These results demonstrate that features other than traumatic axonal injury, such as metabolic insults and hypoxic-ischemic injury secondary to vascular compromise, must contribute to βAPP immunostaining. In addition, we describe a variety of βAPP-immunoreactive structures not previously reported in the pediatric population. This study illustrates that βAPP immunostaining enhances detection of a variety of white matter changes, and provides a basis for interpretation of these results.

Original languageEnglish (US)
Pages (from-to)237-247
Number of pages11
JournalJournal of Neuropathology and Experimental Neurology
Volume62
Issue number3
StatePublished - Mar 1 2003

Fingerprint

Amyloid beta-Protein Precursor
Autopsy
Pediatrics
Staining and Labeling
Near Drowning
Sudden Infant Death
Wounds and Injuries
Motor Vehicles
Carbon Monoxide
Accidents
Asphyxia
Resuscitation
Blood Vessels
Spinal Cord
White Matter
Brain
Population

Keywords

  • Autopsy
  • Beta amyloid precursor protein
  • Brain
  • Immunohistochemistry
  • Pediatric
  • Sudden infant death syndrome (SIDS)

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Beta-amyloid precursor protein staining in nonhomicidal pediatric medicolegal autopsies. / Reichard, R. Ross; White, Charles L.; Hladik, Christa L.; Dolinak, David.

In: Journal of Neuropathology and Experimental Neurology, Vol. 62, No. 3, 01.03.2003, p. 237-247.

Research output: Contribution to journalArticle

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