TY - JOUR
T1 - Beta-amyloid precursor protein staining in nonhomicidal pediatric medicolegal autopsies
AU - Reichard, R. Ross
AU - White, Charles L.
AU - Hladik, Christa L.
AU - Dolinak, David
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2003/3/1
Y1 - 2003/3/1
N2 - Immunohistochemical staining for beta-amyloid precursor protein (βAPP) has been validated as a marker for axonal injury in adults surviving ≥2 hours after white matter damage. The significance of βAPP staining in pediatric brains and spinal cords is not as well established. We evaluated the white matter immunoreactivity for βAPP from a variety of pediatric medicolegal autopsies: natural disease (non-Sudden Infant Death Syndrome [SIDS]), SIDS, motor vehicle accidents, drowning, near-drowning, overlay, carbon monoxide toxicity, miscellaneous trauma, and mechanical asphyxia. The cases of carbon monoxide toxicity, motor vehicle accidents (death at scene), drowning (with resuscitation), and a natural (non-SIDS) death had no significant white matter staining. The traumatic deaths with a significant survival interval, a variety of natural deaths, the near-drowning case. and surprisingly, all SIDS had detectable βAPP white matter immunostaining. These results demonstrate that features other than traumatic axonal injury, such as metabolic insults and hypoxic-ischemic injury secondary to vascular compromise, must contribute to βAPP immunostaining. In addition, we describe a variety of βAPP-immunoreactive structures not previously reported in the pediatric population. This study illustrates that βAPP immunostaining enhances detection of a variety of white matter changes, and provides a basis for interpretation of these results.
AB - Immunohistochemical staining for beta-amyloid precursor protein (βAPP) has been validated as a marker for axonal injury in adults surviving ≥2 hours after white matter damage. The significance of βAPP staining in pediatric brains and spinal cords is not as well established. We evaluated the white matter immunoreactivity for βAPP from a variety of pediatric medicolegal autopsies: natural disease (non-Sudden Infant Death Syndrome [SIDS]), SIDS, motor vehicle accidents, drowning, near-drowning, overlay, carbon monoxide toxicity, miscellaneous trauma, and mechanical asphyxia. The cases of carbon monoxide toxicity, motor vehicle accidents (death at scene), drowning (with resuscitation), and a natural (non-SIDS) death had no significant white matter staining. The traumatic deaths with a significant survival interval, a variety of natural deaths, the near-drowning case. and surprisingly, all SIDS had detectable βAPP white matter immunostaining. These results demonstrate that features other than traumatic axonal injury, such as metabolic insults and hypoxic-ischemic injury secondary to vascular compromise, must contribute to βAPP immunostaining. In addition, we describe a variety of βAPP-immunoreactive structures not previously reported in the pediatric population. This study illustrates that βAPP immunostaining enhances detection of a variety of white matter changes, and provides a basis for interpretation of these results.
KW - Autopsy
KW - Beta amyloid precursor protein
KW - Brain
KW - Immunohistochemistry
KW - Pediatric
KW - Sudden infant death syndrome (SIDS)
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U2 - 10.1093/jnen/62.3.237
DO - 10.1093/jnen/62.3.237
M3 - Article
C2 - 12638728
AN - SCOPUS:0037341141
SN - 0022-3069
VL - 62
SP - 237
EP - 247
JO - Journal of neuropathology and experimental neurology
JF - Journal of neuropathology and experimental neurology
IS - 3
ER -