TY - JOUR
T1 - BHK cell variant with defective fibronectin receptor function
AU - Oppenheimer-Marks, Nancy
AU - Border, Barbara
AU - Grinnell, Frederick
N1 - Funding Information:
ACKNOWLEDGEMENTS. We are indebted to Mr. Doug McAbee and Dr. Bill Snell for their helpful comments regarding this research. This research was supported by a grant from the NIH, CA14609.
PY - 1984/2
Y1 - 1984/2
N2 - Baby hamster kidney cells were mutagenized with N-methyl-N′-nitro-N-nitrosoguanidine and selected to obtain a population of non-attaching cells. The cell variant FN-1 was cloned from the non-attaching cell population, recloned, and tested for cell adhesive interactions using four different assays of fibronectin (pFN) receptor function: cell attachment and spreading on culture dishes and cell binding and phagocytosis of latex beads. On pFN-coated culture dishes, FN-1 cells had decreased attachment compared to parental cells and were unable to spread. With pFN-coated beads, only one third as many pFN-bead binding sites could be detected on FN-1 cells as on the parental cells, and the FN-1 cells were unable to phagocytose the pFN-coated beads. In other studies, the variant cells were able to attach normally and spread partially on substrata coated with polycationic ferritin, concanavalin A, or anti-BHK cell surface antibody. The results suggest that the pFN-receptor function of FN-1 cells is defective.
AB - Baby hamster kidney cells were mutagenized with N-methyl-N′-nitro-N-nitrosoguanidine and selected to obtain a population of non-attaching cells. The cell variant FN-1 was cloned from the non-attaching cell population, recloned, and tested for cell adhesive interactions using four different assays of fibronectin (pFN) receptor function: cell attachment and spreading on culture dishes and cell binding and phagocytosis of latex beads. On pFN-coated culture dishes, FN-1 cells had decreased attachment compared to parental cells and were unable to spread. With pFN-coated beads, only one third as many pFN-bead binding sites could be detected on FN-1 cells as on the parental cells, and the FN-1 cells were unable to phagocytose the pFN-coated beads. In other studies, the variant cells were able to attach normally and spread partially on substrata coated with polycationic ferritin, concanavalin A, or anti-BHK cell surface antibody. The results suggest that the pFN-receptor function of FN-1 cells is defective.
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U2 - 10.1016/0309-1651(84)90084-5
DO - 10.1016/0309-1651(84)90084-5
M3 - Article
C2 - 6325027
AN - SCOPUS:0021379246
SN - 0309-1651
VL - 8
SP - 171
EP - 178
JO - Cell Biology International Reports
JF - Cell Biology International Reports
IS - 2
ER -