Bifunctional atrial natriuretic peptide receptor (type a) exists as a disulfide-linked tetramer in plasma membranes of bovine adrenal cortex

Tomoko Iwata, Keiko Uchida-mizuno, Takeshi Katafuchi, Teizo Ito, Hiromi Hagiwara, Shigehisa Hirose

Research output: Contribution to journalArticle

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Abstract

Type A atrial natriuretic peptide (ANP) receptor was demonstrated to be present as a tetramer in the bovine adrenal cortex. Type A ANP receptor is composed of two functional domains, namely extracellular ANP-binding and cytoplasmic guanylate cyclase domains, and generally considered to be present as a single polypeptide chain of about 140 kDa based on its primary structure deduced from the cDNA sequence and its SDS/PAGE profile under reducing conditions. Characterization of the type A receptor or receptor/cyclase under non-reducing conditions led to the discovery stated in the title. The type A ANP receptor was partially purified from bovine adrenal cortex membranes by Blue-Sepharose and GTP-agarose chromatography. SDS-PAGE analysis of the receptor preparation revealed that although under reducing conditions it migrated as a 140-kDa band, the mobility of the receptor was greatly retarded in the absence of reducing agents, suggesting that the type A ANP receptor is present as a disulfide-linked oligomer in its native state. Further analysis using SDS-polyacrylamide-agarose gels suitable for determining the sizes of high-molecular-weight proteins revealed that the oligomer has an Mr of 500,000-550,000. This result clearly indicates that the native form of the type A receptor is a tetramer composed of four 140-kDa disulfide-linked receptor/cyclase molecules.

Original languageEnglish (US)
Pages (from-to)35-39
Number of pages5
JournalJournal of Biochemistry
Volume110
Issue number1
StatePublished - Jul 1991

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Natriuretic Peptides
Atrial Natriuretic Factor Receptors
Plasma Membrane
Adrenal Cortex
Cell membranes
Cortex
Disulfides
Receptor
Cell Membrane
Oligomers
Polyacrylamide Gel Electrophoresis
Agarose Chromatography
Guanylate Cyclase
Reducing Agents
Atrial Natriuretic Factor
Chromatography
Sepharose
Polypeptides
Reducing agents
Polyacrylates

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Bifunctional atrial natriuretic peptide receptor (type a) exists as a disulfide-linked tetramer in plasma membranes of bovine adrenal cortex. / Iwata, Tomoko; Uchida-mizuno, Keiko; Katafuchi, Takeshi; Ito, Teizo; Hagiwara, Hiromi; Hirose, Shigehisa.

In: Journal of Biochemistry, Vol. 110, No. 1, 07.1991, p. 35-39.

Research output: Contribution to journalArticle

Iwata, T, Uchida-mizuno, K, Katafuchi, T, Ito, T, Hagiwara, H & Hirose, S 1991, 'Bifunctional atrial natriuretic peptide receptor (type a) exists as a disulfide-linked tetramer in plasma membranes of bovine adrenal cortex', Journal of Biochemistry, vol. 110, no. 1, pp. 35-39.
Iwata, Tomoko ; Uchida-mizuno, Keiko ; Katafuchi, Takeshi ; Ito, Teizo ; Hagiwara, Hiromi ; Hirose, Shigehisa. / Bifunctional atrial natriuretic peptide receptor (type a) exists as a disulfide-linked tetramer in plasma membranes of bovine adrenal cortex. In: Journal of Biochemistry. 1991 ; Vol. 110, No. 1. pp. 35-39.
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