TY - JOUR
T1 - Bilayer measurement of endoplasmic reticulum Ca2+ channels
AU - Bezprozvanny, Ilya
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2013/11
Y1 - 2013/11
N2 - Reconstitution of ion channels into planar lipid bilayers (also called black lipid membranes or BLM) is the most widely used method to conduct physiological studies of intracellular ion channels, including endoplasmic reticulum (ER) calcium (Ca2+) channels. The two main types of Ca2+ release channels in the ER membrane are ryanodine receptors (RyanRs) and inositol(1,4,5)-trisphosphate receptors (InsP3Rs). Use of the BLM reconstitution technique enabled the initial description of the functional properties of InsP3R and RyanR at the single-channel level more than 20 years ago. Since then, BLM reconstitution methods have been used to study physiological modulation and to perform structure- function analysis of these channels, and to study pathological changes in the function of InsP3R and RyanR in various disease states. The BLM technique has also been useful for studies of other intracellular Ca2+ channels, such as ER Ca2+ leak presenilin channels and NAADP-gated lysosomal Ca2+ channels encoded by TPC2. In this article, basic protocols used for BLM studies of ER Ca2+ channels are introduced.
AB - Reconstitution of ion channels into planar lipid bilayers (also called black lipid membranes or BLM) is the most widely used method to conduct physiological studies of intracellular ion channels, including endoplasmic reticulum (ER) calcium (Ca2+) channels. The two main types of Ca2+ release channels in the ER membrane are ryanodine receptors (RyanRs) and inositol(1,4,5)-trisphosphate receptors (InsP3Rs). Use of the BLM reconstitution technique enabled the initial description of the functional properties of InsP3R and RyanR at the single-channel level more than 20 years ago. Since then, BLM reconstitution methods have been used to study physiological modulation and to perform structure- function analysis of these channels, and to study pathological changes in the function of InsP3R and RyanR in various disease states. The BLM technique has also been useful for studies of other intracellular Ca2+ channels, such as ER Ca2+ leak presenilin channels and NAADP-gated lysosomal Ca2+ channels encoded by TPC2. In this article, basic protocols used for BLM studies of ER Ca2+ channels are introduced.
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U2 - 10.1101/pdb.top066225
DO - 10.1101/pdb.top066225
M3 - Article
C2 - 24184754
AN - SCOPUS:84887045501
VL - 2013
SP - 1012
EP - 1016
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1559-6095
IS - 11
ER -