Bile salt hydrophobicity influences cholesterol recruitment from rat liver in vivo when cholesterol synthesis and lipoprotein uptake are constant

L. E. Bilhartz, J. M. Dietschy

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

These studies were undertaken to characterize the role of bile salt hydrophobicity in determining the rate of cholesterol recruitment from the liver. Using an in vivo rat model in which the acquisition of hepatic cholesterol from chylomicron remnants, low-density lipoproteins, and de novo synthesis was measured and kept constant, it was found that the amount of sterol recruited from the liver cell increased progressively as the liver was probed with a constant infusion of progressively more hydrophobic bile salts. The absolute secretion rate of both cholesterol and phospholipid increased nearly 50% as the hydrophobic index of the bile salts traversing the liver is increased from 1.7 to 4.5, but the ratio of cholesterol to phospolipid secreted in bile remained nearly constant. Thus, when cholesterol entry into the hepatocyte via lipoproteins and de novo cholesterol synthesis is constant, the mass of cellular cholesterol recruited into the bile is directly proportional to the hydrophobic-hydrophilic balance of the secreted bile salts.

Original languageEnglish (US)
Pages (from-to)771-779
Number of pages9
JournalGastroenterology
Volume95
Issue number3
StatePublished - 1988

Fingerprint

Bile Acids and Salts
Hydrophobic and Hydrophilic Interactions
Cholesterol
Liver
Bile
Chylomicron Remnants
Sterols
lipoprotein cholesterol
LDL Lipoproteins
Lipoproteins
Hepatocytes
Phospholipids

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Bile salt hydrophobicity influences cholesterol recruitment from rat liver in vivo when cholesterol synthesis and lipoprotein uptake are constant. / Bilhartz, L. E.; Dietschy, J. M.

In: Gastroenterology, Vol. 95, No. 3, 1988, p. 771-779.

Research output: Contribution to journalArticle

@article{d6369b77f9cc41eeb7afc956be1f873e,
title = "Bile salt hydrophobicity influences cholesterol recruitment from rat liver in vivo when cholesterol synthesis and lipoprotein uptake are constant",
abstract = "These studies were undertaken to characterize the role of bile salt hydrophobicity in determining the rate of cholesterol recruitment from the liver. Using an in vivo rat model in which the acquisition of hepatic cholesterol from chylomicron remnants, low-density lipoproteins, and de novo synthesis was measured and kept constant, it was found that the amount of sterol recruited from the liver cell increased progressively as the liver was probed with a constant infusion of progressively more hydrophobic bile salts. The absolute secretion rate of both cholesterol and phospholipid increased nearly 50{\%} as the hydrophobic index of the bile salts traversing the liver is increased from 1.7 to 4.5, but the ratio of cholesterol to phospolipid secreted in bile remained nearly constant. Thus, when cholesterol entry into the hepatocyte via lipoproteins and de novo cholesterol synthesis is constant, the mass of cellular cholesterol recruited into the bile is directly proportional to the hydrophobic-hydrophilic balance of the secreted bile salts.",
author = "Bilhartz, {L. E.} and Dietschy, {J. M.}",
year = "1988",
language = "English (US)",
volume = "95",
pages = "771--779",
journal = "Gastroenterology",
issn = "0016-5085",
publisher = "W.B. Saunders Ltd",
number = "3",

}

TY - JOUR

T1 - Bile salt hydrophobicity influences cholesterol recruitment from rat liver in vivo when cholesterol synthesis and lipoprotein uptake are constant

AU - Bilhartz, L. E.

AU - Dietschy, J. M.

PY - 1988

Y1 - 1988

N2 - These studies were undertaken to characterize the role of bile salt hydrophobicity in determining the rate of cholesterol recruitment from the liver. Using an in vivo rat model in which the acquisition of hepatic cholesterol from chylomicron remnants, low-density lipoproteins, and de novo synthesis was measured and kept constant, it was found that the amount of sterol recruited from the liver cell increased progressively as the liver was probed with a constant infusion of progressively more hydrophobic bile salts. The absolute secretion rate of both cholesterol and phospholipid increased nearly 50% as the hydrophobic index of the bile salts traversing the liver is increased from 1.7 to 4.5, but the ratio of cholesterol to phospolipid secreted in bile remained nearly constant. Thus, when cholesterol entry into the hepatocyte via lipoproteins and de novo cholesterol synthesis is constant, the mass of cellular cholesterol recruited into the bile is directly proportional to the hydrophobic-hydrophilic balance of the secreted bile salts.

AB - These studies were undertaken to characterize the role of bile salt hydrophobicity in determining the rate of cholesterol recruitment from the liver. Using an in vivo rat model in which the acquisition of hepatic cholesterol from chylomicron remnants, low-density lipoproteins, and de novo synthesis was measured and kept constant, it was found that the amount of sterol recruited from the liver cell increased progressively as the liver was probed with a constant infusion of progressively more hydrophobic bile salts. The absolute secretion rate of both cholesterol and phospholipid increased nearly 50% as the hydrophobic index of the bile salts traversing the liver is increased from 1.7 to 4.5, but the ratio of cholesterol to phospolipid secreted in bile remained nearly constant. Thus, when cholesterol entry into the hepatocyte via lipoproteins and de novo cholesterol synthesis is constant, the mass of cellular cholesterol recruited into the bile is directly proportional to the hydrophobic-hydrophilic balance of the secreted bile salts.

UR - http://www.scopus.com/inward/record.url?scp=0023805878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023805878&partnerID=8YFLogxK

M3 - Article

VL - 95

SP - 771

EP - 779

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 3

ER -