Biliary bile acids in primary biliary cirrhosis

Effect of ursodeoxycholic acid

B. Combes, Jr Carithers R.L., W. C. Maddrey, S. Munoz, G. Garcia-Tsao, G. F. Bonner, J. L. Boyer, V. A. Luketic, M. L. Shiffman, M. G. Peters, H. White, R. K. Zetterman, R. Risser, S. S. Rossi, A. F. Hofmann

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Bile acid composition in fasting duodenal bile was assessed at entry and at 2 years in patients with primary biliary cirrhosis (PBC) enrolled in a randomized, double-blind, placebo-controlled trial of ursodeoxycholic acid (UDCA) (10-12 mg/kg/d) taken as a single bedtime dose. Specimens were analyzed by a high-pressure liquid chromatography method that had been validated against gas chromatography. Percent composition in bile (mean ± SD) for 98 patients at entry for cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA), and ursodeoxycholic (UDCA) acids, respectively, were 57.4 ± 18.6, 31.5 ± 15.5, 8.0 ± 9.3, 0.3 ± 1.0, and 0.6 ± 0.9. Values for CA were increased, whereas those for CDCA, DCA, LCA, and UDCA were decreased when compared with values in normal persons. Bile acid composition of the major bile acids did not change after 2 years on placebo medication. By contrast, in patients receiving UDCA for 2 years, bile became enriched with UDCA on average to 40.1%, and significant decreases were noted for CA (to 32.2%) and CDCA (to 19.5%). No change in percent composition was observed for DCA and LCA. Percent composition at entry and changes in composition after 2 years on UDCA were similar in patients with varying severity of PBC. In patients whose bile was not enriched in UDCA (entry and placebo-treated specimens), CA, CDCA, DCA, and the small amount of UDCA found in some of these specimens were conjugated to a greater extent with glycine (52%-64%) than with taurine (36%-48%). Treatment with UDCA caused the proportion of all endogenous bile acids conjugated with glycine to increase to 69% to 78%, while the proportion conjugated with taurine (22%-31%) fell (P < .05). Administered UDCA was also conjugated predominantly with glycine (87%).

Original languageEnglish (US)
Pages (from-to)1649-1654
Number of pages6
JournalHepatology
Volume29
Issue number6
DOIs
StatePublished - 1999

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Ursodeoxycholic Acid
Biliary Liver Cirrhosis
Bile Acids and Salts
Bile
Glycine
Taurine
Placebos
Gas Chromatography
Fasting
Reference Values
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Hepatology

Cite this

Combes, B., Carithers R.L., J., Maddrey, W. C., Munoz, S., Garcia-Tsao, G., Bonner, G. F., ... Hofmann, A. F. (1999). Biliary bile acids in primary biliary cirrhosis: Effect of ursodeoxycholic acid. Hepatology, 29(6), 1649-1654. https://doi.org/10.1002/hep.510290618

Biliary bile acids in primary biliary cirrhosis : Effect of ursodeoxycholic acid. / Combes, B.; Carithers R.L., Jr; Maddrey, W. C.; Munoz, S.; Garcia-Tsao, G.; Bonner, G. F.; Boyer, J. L.; Luketic, V. A.; Shiffman, M. L.; Peters, M. G.; White, H.; Zetterman, R. K.; Risser, R.; Rossi, S. S.; Hofmann, A. F.

In: Hepatology, Vol. 29, No. 6, 1999, p. 1649-1654.

Research output: Contribution to journalArticle

Combes, B, Carithers R.L., J, Maddrey, WC, Munoz, S, Garcia-Tsao, G, Bonner, GF, Boyer, JL, Luketic, VA, Shiffman, ML, Peters, MG, White, H, Zetterman, RK, Risser, R, Rossi, SS & Hofmann, AF 1999, 'Biliary bile acids in primary biliary cirrhosis: Effect of ursodeoxycholic acid', Hepatology, vol. 29, no. 6, pp. 1649-1654. https://doi.org/10.1002/hep.510290618
Combes B, Carithers R.L. J, Maddrey WC, Munoz S, Garcia-Tsao G, Bonner GF et al. Biliary bile acids in primary biliary cirrhosis: Effect of ursodeoxycholic acid. Hepatology. 1999;29(6):1649-1654. https://doi.org/10.1002/hep.510290618
Combes, B. ; Carithers R.L., Jr ; Maddrey, W. C. ; Munoz, S. ; Garcia-Tsao, G. ; Bonner, G. F. ; Boyer, J. L. ; Luketic, V. A. ; Shiffman, M. L. ; Peters, M. G. ; White, H. ; Zetterman, R. K. ; Risser, R. ; Rossi, S. S. ; Hofmann, A. F. / Biliary bile acids in primary biliary cirrhosis : Effect of ursodeoxycholic acid. In: Hepatology. 1999 ; Vol. 29, No. 6. pp. 1649-1654.
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abstract = "Bile acid composition in fasting duodenal bile was assessed at entry and at 2 years in patients with primary biliary cirrhosis (PBC) enrolled in a randomized, double-blind, placebo-controlled trial of ursodeoxycholic acid (UDCA) (10-12 mg/kg/d) taken as a single bedtime dose. Specimens were analyzed by a high-pressure liquid chromatography method that had been validated against gas chromatography. Percent composition in bile (mean ± SD) for 98 patients at entry for cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA), and ursodeoxycholic (UDCA) acids, respectively, were 57.4 ± 18.6, 31.5 ± 15.5, 8.0 ± 9.3, 0.3 ± 1.0, and 0.6 ± 0.9. Values for CA were increased, whereas those for CDCA, DCA, LCA, and UDCA were decreased when compared with values in normal persons. Bile acid composition of the major bile acids did not change after 2 years on placebo medication. By contrast, in patients receiving UDCA for 2 years, bile became enriched with UDCA on average to 40.1{\%}, and significant decreases were noted for CA (to 32.2{\%}) and CDCA (to 19.5{\%}). No change in percent composition was observed for DCA and LCA. Percent composition at entry and changes in composition after 2 years on UDCA were similar in patients with varying severity of PBC. In patients whose bile was not enriched in UDCA (entry and placebo-treated specimens), CA, CDCA, DCA, and the small amount of UDCA found in some of these specimens were conjugated to a greater extent with glycine (52{\%}-64{\%}) than with taurine (36{\%}-48{\%}). Treatment with UDCA caused the proportion of all endogenous bile acids conjugated with glycine to increase to 69{\%} to 78{\%}, while the proportion conjugated with taurine (22{\%}-31{\%}) fell (P < .05). Administered UDCA was also conjugated predominantly with glycine (87{\%}).",
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AU - Garcia-Tsao, G.

AU - Bonner, G. F.

AU - Boyer, J. L.

AU - Luketic, V. A.

AU - Shiffman, M. L.

AU - Peters, M. G.

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AU - Hofmann, A. F.

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