TY - JOUR
T1 - Binding of plasma fibronectin to the surfaces of BHK cells in suspension at 4 °C
AU - Grinnell, Frederick
AU - Lang, Billie R.
AU - Phan, Trung V.
N1 - Funding Information:
This work received financial support from the Intema-tional Development Research Centre (IDRC) of Canada (3-P-79-0132). N. T. received a scholarship from Faculty of Graduate Studies, Mahidol University. We thank Thitika Vajrodaya for drawing of figures and Urai Sajjaharutai for typing the manuscript.
Funding Information:
We are indebtedt o Drs Bill Snell and Nancy Oppenheimer-Marksf or their helpful discussionsd uring the course of these studies and with preparation of the manuscript.D r Leon Eidels also assisted with preparation of the manuscript. These research was supported by a grant from the NIH, CA14609.
PY - 1982/12
Y1 - 1982/12
N2 - Plasma fibronectin is shown by several different criteria to bind to suspended BHK cells if the binding incubations are carried out at 4 °C. In indirect immunofluorescence experiments, fibronectin bound to suspended BHK cells at 4 °C in a punctate distribution over the entire cell surfaces. Little binding, however, was detected on cells incubated with fibronectin at 37 °C. The fibronectin bound to the cells at 4 °C was functionally active, since these cells subsequently were able to spread on tissue culture dishes in protein-free medium, unlike cells preincubated with fibronectin at 37 °C or in the absence of fibronectin. Also, the cell surface receptors for soluble fibronectin and fibronectin-coated beads appeared to be similar, since cells preincubated with fibronectin at 4 °C subsequently bound fewer fibronectin-coated beads than control cells. In biochemical studies with radiolabeled fibronectin, binding of fibronectin to the cells was shown to increase with incubation time up to 4 h. In competition experiments with unlabeled fibronectin, 30% of the binding of radiolabeled fibronectin could be inhibited.
AB - Plasma fibronectin is shown by several different criteria to bind to suspended BHK cells if the binding incubations are carried out at 4 °C. In indirect immunofluorescence experiments, fibronectin bound to suspended BHK cells at 4 °C in a punctate distribution over the entire cell surfaces. Little binding, however, was detected on cells incubated with fibronectin at 37 °C. The fibronectin bound to the cells at 4 °C was functionally active, since these cells subsequently were able to spread on tissue culture dishes in protein-free medium, unlike cells preincubated with fibronectin at 37 °C or in the absence of fibronectin. Also, the cell surface receptors for soluble fibronectin and fibronectin-coated beads appeared to be similar, since cells preincubated with fibronectin at 4 °C subsequently bound fewer fibronectin-coated beads than control cells. In biochemical studies with radiolabeled fibronectin, binding of fibronectin to the cells was shown to increase with incubation time up to 4 h. In competition experiments with unlabeled fibronectin, 30% of the binding of radiolabeled fibronectin could be inhibited.
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U2 - 10.1016/0014-4827(82)90398-6
DO - 10.1016/0014-4827(82)90398-6
M3 - Article
C2 - 6293857
AN - SCOPUS:0020448801
SN - 0014-4827
VL - 142
SP - 499
EP - 504
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -