Binding site specificity and factor redundancy in activator protein-1-driven human papillomavirus chromatin-dependent transcription

Wei Ming Wang, Shwu Yuan Wu, A. Young Lee, Cheng Ming Chiang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Activator protein-1 (AP-1) regulates diverse gene responses triggered by environmental cues and virus-induced cellular stress. Although many signaling events leading to AP-1 activation have been described, the fundamental features underlying binding site selection and factor recruitment of dimeric AP-1 complexes to their target genes remain mostly uncharacterized. Using recombinant full-length human AP-1 dimers formed between c-Jun and Fos family members (c-Fos, FosB, Fra-1, Fra-2) for DNA binding and transcriptional analysis, we found that each of these AP-1 complex exhibits differential activity for distinct non-consensus AP-1 sites present in human papillomavirus (HPV), and each AP-1 complex is capable of activating transcription from in vitro-reconstituted HPV chromatin in a p300- and acetyl-CoA-dependent manner. Transcription from HPV chromatin requires AP-1-dependent and contact-driven recruitment of p300. Acetylation of dimeric AP-1 complexes by p300 enhances AP-1 binding to DNA. Using a human C-33A cervical cancer-derived cell line harboring the episomal HPV type 11 genome, we illustrate binding site selectivity recognized by c-Jun, JunB, JunD, and various Fos family members in a combinatorial and unique pattern, highlighting the diversity and importance of non-canonical binding site recognition by various AP-1 family proteins.

Original languageEnglish (US)
Pages (from-to)40974-40986
Number of pages13
JournalJournal of Biological Chemistry
Volume286
Issue number47
DOIs
StatePublished - Nov 25 2011

Fingerprint

Transcription Factor AP-1
Transcription
Chromatin
Redundancy
Binding Sites
Genes
Human papillomavirus 11
Acetylation
Site selection
Acetyl Coenzyme A
DNA
Viruses
Protein Binding
Uterine Cervical Neoplasms
Dimers
Cues
Chemical activation
Cells
Genome
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Binding site specificity and factor redundancy in activator protein-1-driven human papillomavirus chromatin-dependent transcription. / Wang, Wei Ming; Wu, Shwu Yuan; Lee, A. Young; Chiang, Cheng Ming.

In: Journal of Biological Chemistry, Vol. 286, No. 47, 25.11.2011, p. 40974-40986.

Research output: Contribution to journalArticle

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