Bioavailability of cadmium and biochemical responses on the freshwater bivalve Corbicula fluminea - the role of TiO2 nanoparticles

Gonçalo Vale, Cristiana Franco, Mário S. Diniz, Margarida M.C.dos Santos, Rute F. Domingos

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The increasing and widespread applications of TiO2 engineered nanoparticles (nTiO2) led to the release of these materials into aquatic environments and consequently a change on the assessment of the environmental risk of trace metals. In this work, the role of two commercial nTiO2 with distinct crystalline phases and sizes (nTiO2-P25: 80% anatase+20% rutile, d=20nm; nTiO2-NA: 100% anatase, d=5nm; 0.1 and 1.0mgL-1) on Cd (112μgL-1) speciation, biouptake and toxicity for the freshwater bivalve Corbicula fluminea was evaluated. The electroanalytical technique 'absence of gradients and Nernstian equilibrium stripping (AGNES)' was used to quantify the free Cd concentrations in the exposure medium in presence of both particles. Despite ca. 30-40% decrease of free Cd in the medium in presence of nTiO2, Cd uptake by C. fluminea was similar in the absence and presence of either of the particles. Superoxide dismutase and glutathione-S-transferase activities remained unchanged for Cd in absence and presence of nTiO2, whereas a significant increase of the catalase activity was obtained at the third day for Cd in presence of both nTiO2. Despite lipid peroxidation data shows that the presence of both nTiO2 seems to exert cells damage, a more quantitative description is not possible with the obtained data. The lack of clear-cut responses by the studied biomarkers, even when only in presence of Cd, do not allow insights into the effect of the presence of nTiO2 on the Cd toxicity to the bivalves. Notwithstanding, morphological changes in the digestive gland were clearly obtained in the presence of Cd, nTiO2 and Cd+nTiO2 indicating an inflammatory response.

Original languageEnglish (US)
Pages (from-to)161-168
Number of pages8
JournalEcotoxicology and Environmental Safety
Volume109
DOIs
StatePublished - Nov 1 2014

Fingerprint

Corbicula
Bivalvia
Fresh Water
Cadmium
Nanoparticles
Titanium dioxide
Biological Availability
Toxicity
Biomarkers
Lipids
Cells
Crystalline materials
Glutathione Transferase
Catalase
Lipid Peroxidation
Superoxide Dismutase
Metals
titanium dioxide

Keywords

  • Bioavailability
  • Biomarkers
  • Cadmium
  • Corbicula fluminea
  • Speciation
  • TiO nanoparticles

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Bioavailability of cadmium and biochemical responses on the freshwater bivalve Corbicula fluminea - the role of TiO2 nanoparticles. / Vale, Gonçalo; Franco, Cristiana; Diniz, Mário S.; Santos, Margarida M.C.dos; Domingos, Rute F.

In: Ecotoxicology and Environmental Safety, Vol. 109, 01.11.2014, p. 161-168.

Research output: Contribution to journalArticle

Vale, Gonçalo ; Franco, Cristiana ; Diniz, Mário S. ; Santos, Margarida M.C.dos ; Domingos, Rute F. / Bioavailability of cadmium and biochemical responses on the freshwater bivalve Corbicula fluminea - the role of TiO2 nanoparticles. In: Ecotoxicology and Environmental Safety. 2014 ; Vol. 109. pp. 161-168.
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