Biochemical analysis of an MHC-linked hematopoietic cell surface antigen, Qa-2

M. J. Soloski, E. S. Vitetta, L. Flaherty, J. W. Uhr

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The region of the murine 17th chromosome telomeric to H-2D encodes a group of serologically defined cell surface antigens termed Qa-1-5. These antigens are of interest because their expression is restricted to hematopoietic cells. In addition, the molecular weight and subunit structure (ie, association with β-2 microglobulin) of Qa-2 molecules are similar to H-2 and TL antigens. In the present studies, we have prepared isotopically labeled Qa-2 and H-2 molecules from mitogen-stimulated C57BL/6 spleen cells. Comparative peptide mapping of tryptic peptides from Qa-2 and H-2 molecules (K(b), D(b)K(k), D(d)) reveal that Qa-2 has a unique primary structure. However, considerable homology is indicated since 30-40% of the Qa-2 peptides cochromatograph with peptides derived from H-2K(b), H-2D(b), H-2K(k), and H-2D(d). Studies by other investigators have demonstrated that similar levels of structural homology are observed when H-2K, H-2D, and H-2L tryptic peptides are analyzed. We conclude from these studies that the Qa-2 alloantigen is structurally related to a class of cell surface molecules (ie, H-2) that play critical roles in immune recognition processes. These data further suggest that the genes encoding Qa-2 and H-2 molecules have arisen from a common primordial gene.

Original languageEnglish (US)
Title of host publicationJournal of Supramolecular and Cellular Biochemistry
Pages167-177
Number of pages11
Volume16
Edition2
StatePublished - 1981

ASJC Scopus subject areas

  • Biochemistry

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    Soloski, M. J., Vitetta, E. S., Flaherty, L., & Uhr, J. W. (1981). Biochemical analysis of an MHC-linked hematopoietic cell surface antigen, Qa-2. In Journal of Supramolecular and Cellular Biochemistry (2 ed., Vol. 16, pp. 167-177)