Biochemical analysis of Tankyrase activity in zebrafish in vitro and in vivo

Jesung Moon, James F. Amatruda

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The activity of tankyrase (Tnks) enzymes modulates the activity of the β-catenin destruction complex in the Wnt/β-catenin signaling pathway. Here, we describe a method for determining the accessibility of various zebrafish tissues in vivo and in vitro to small molecule inhibitors of Tankyrase enzymes. This biochemical assay will facilitate chemically based studies focused on understanding the role of Tankyrase in cell fate reprogramming and tissue homeostasis and provide insights into the potential role of Wnt/β-catenin signaling in these processes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages95-100
Number of pages6
Volume1481
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1481
ISSN (Print)10643745

Fingerprint

Tankyrases
Catenins
Zebrafish
Wnt Signaling Pathway
Enzyme Inhibitors
Homeostasis
Enzymes
In Vitro Techniques

Keywords

  • Tankyrase
  • Western blotting
  • Wnt inhibitor
  • Wnt/β-catenin signaling
  • Zebrafish

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Genetics

Cite this

Moon, J., & Amatruda, J. F. (2016). Biochemical analysis of Tankyrase activity in zebrafish in vitro and in vivo. In Methods in Molecular Biology (Vol. 1481, pp. 95-100). (Methods in Molecular Biology; Vol. 1481). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6393-5_10

Biochemical analysis of Tankyrase activity in zebrafish in vitro and in vivo. / Moon, Jesung; Amatruda, James F.

Methods in Molecular Biology. Vol. 1481 Humana Press Inc., 2016. p. 95-100 (Methods in Molecular Biology; Vol. 1481).

Research output: Chapter in Book/Report/Conference proceedingChapter

Moon, J & Amatruda, JF 2016, Biochemical analysis of Tankyrase activity in zebrafish in vitro and in vivo. in Methods in Molecular Biology. vol. 1481, Methods in Molecular Biology, vol. 1481, Humana Press Inc., pp. 95-100. https://doi.org/10.1007/978-1-4939-6393-5_10
Moon J, Amatruda JF. Biochemical analysis of Tankyrase activity in zebrafish in vitro and in vivo. In Methods in Molecular Biology. Vol. 1481. Humana Press Inc. 2016. p. 95-100. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6393-5_10
Moon, Jesung ; Amatruda, James F. / Biochemical analysis of Tankyrase activity in zebrafish in vitro and in vivo. Methods in Molecular Biology. Vol. 1481 Humana Press Inc., 2016. pp. 95-100 (Methods in Molecular Biology).
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