Biochemical effects of dipyridamole on purine overproduction and excretion by mutant murine T-lymphoblasts

B. Ullman, K. Kaur

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A mutant murine T-cell line which overproduces purines and excretes massive quantities of inosine into the culture medium has served as a cell culture model for overproduction hyperuricemia. Incubation of these cells with micromolar concentrations of dipyridamole, a potent inhibitor of nucleoside transport, prevents the excretion of inosine and depresses the rate of purine biosynthesis to that of wild type cells. These concentrations of dipyridamole have no effect on cellular growth rate or on the intracellular nucleoside triphosphate or phosphoribosylpyrophosphate pools. We suggest that dipyridamole might also be useful in ameliorating purine overproduction associated with hyperuricemia and gout.

Original languageEnglish (US)
Pages (from-to)9620-9622
Number of pages3
JournalJournal of Biological Chemistry
Volume258
Issue number16
StatePublished - 1983

Fingerprint

Dipyridamole
Inosine
Hyperuricemia
Nucleosides
Purines
T-cells
Gout
Biosynthesis
Cell culture
Culture Media
Cell Culture Techniques
Cells
T-Lymphocytes
Cell Line
Growth
purine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Biochemical effects of dipyridamole on purine overproduction and excretion by mutant murine T-lymphoblasts. / Ullman, B.; Kaur, K.

In: Journal of Biological Chemistry, Vol. 258, No. 16, 1983, p. 9620-9622.

Research output: Contribution to journalArticle

@article{df49c25866b04938b4da9a406818d344,
title = "Biochemical effects of dipyridamole on purine overproduction and excretion by mutant murine T-lymphoblasts",
abstract = "A mutant murine T-cell line which overproduces purines and excretes massive quantities of inosine into the culture medium has served as a cell culture model for overproduction hyperuricemia. Incubation of these cells with micromolar concentrations of dipyridamole, a potent inhibitor of nucleoside transport, prevents the excretion of inosine and depresses the rate of purine biosynthesis to that of wild type cells. These concentrations of dipyridamole have no effect on cellular growth rate or on the intracellular nucleoside triphosphate or phosphoribosylpyrophosphate pools. We suggest that dipyridamole might also be useful in ameliorating purine overproduction associated with hyperuricemia and gout.",
author = "B. Ullman and K. Kaur",
year = "1983",
language = "English (US)",
volume = "258",
pages = "9620--9622",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "16",

}

TY - JOUR

T1 - Biochemical effects of dipyridamole on purine overproduction and excretion by mutant murine T-lymphoblasts

AU - Ullman, B.

AU - Kaur, K.

PY - 1983

Y1 - 1983

N2 - A mutant murine T-cell line which overproduces purines and excretes massive quantities of inosine into the culture medium has served as a cell culture model for overproduction hyperuricemia. Incubation of these cells with micromolar concentrations of dipyridamole, a potent inhibitor of nucleoside transport, prevents the excretion of inosine and depresses the rate of purine biosynthesis to that of wild type cells. These concentrations of dipyridamole have no effect on cellular growth rate or on the intracellular nucleoside triphosphate or phosphoribosylpyrophosphate pools. We suggest that dipyridamole might also be useful in ameliorating purine overproduction associated with hyperuricemia and gout.

AB - A mutant murine T-cell line which overproduces purines and excretes massive quantities of inosine into the culture medium has served as a cell culture model for overproduction hyperuricemia. Incubation of these cells with micromolar concentrations of dipyridamole, a potent inhibitor of nucleoside transport, prevents the excretion of inosine and depresses the rate of purine biosynthesis to that of wild type cells. These concentrations of dipyridamole have no effect on cellular growth rate or on the intracellular nucleoside triphosphate or phosphoribosylpyrophosphate pools. We suggest that dipyridamole might also be useful in ameliorating purine overproduction associated with hyperuricemia and gout.

UR - http://www.scopus.com/inward/record.url?scp=0020561705&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020561705&partnerID=8YFLogxK

M3 - Article

VL - 258

SP - 9620

EP - 9622

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 16

ER -