TY - JOUR
T1 - Biochemical methods used to study the gene expression and protein complexes in the filamentous fungus Neurospora crassa.
AU - Guo, Jinhu
AU - Huang, Guocun
AU - Cha, Joonseok
AU - Liu, Y.
PY - 2010
Y1 - 2010
N2 - Biochemical approaches are powerful tools for investigating mechanisms of biological processes. Here, we describe several biochemical approaches that have been successfully in our laboratory to study the filamentous fungus Neurospora crassa. These approaches include protein extraction and western blot analysis, protein purification using epitope-tagged fusion protein, protein immunoprecipitation (IP) and Chromatin Immunoprecipitation (ChIP) assays. These methods can also be modified for use in other filamentous fungi.
AB - Biochemical approaches are powerful tools for investigating mechanisms of biological processes. Here, we describe several biochemical approaches that have been successfully in our laboratory to study the filamentous fungus Neurospora crassa. These approaches include protein extraction and western blot analysis, protein purification using epitope-tagged fusion protein, protein immunoprecipitation (IP) and Chromatin Immunoprecipitation (ChIP) assays. These methods can also be modified for use in other filamentous fungi.
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U2 - 10.1007/978-1-60761-611-5_14
DO - 10.1007/978-1-60761-611-5_14
M3 - Article
C2 - 20238270
AN - SCOPUS:77953639605
SN - 1064-3745
VL - 638
SP - 189
EP - 200
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -