Biochemical methods used to study the gene expression and protein complexes in the filamentous fungus Neurospora crassa.

Jinhu Guo, Guocun Huang, Joonseok Cha, Y. Liu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Biochemical approaches are powerful tools for investigating mechanisms of biological processes. Here, we describe several biochemical approaches that have been successfully in our laboratory to study the filamentous fungus Neurospora crassa. These approaches include protein extraction and western blot analysis, protein purification using epitope-tagged fusion protein, protein immunoprecipitation (IP) and Chromatin Immunoprecipitation (ChIP) assays. These methods can also be modified for use in other filamentous fungi.

Original languageEnglish (US)
Pages (from-to)189-200
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume638
DOIs
StatePublished - 2010

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Neurospora crassa
Fungi
Gene Expression
Proteins
Biological Phenomena
Chromatin Immunoprecipitation
Immunoprecipitation
Epitopes
Western Blotting

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Biochemical methods used to study the gene expression and protein complexes in the filamentous fungus Neurospora crassa. / Guo, Jinhu; Huang, Guocun; Cha, Joonseok; Liu, Y.

In: Methods in molecular biology (Clifton, N.J.), Vol. 638, 2010, p. 189-200.

Research output: Contribution to journalArticle

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