Biochemical modulation of radiation-induced apoptosis in murine lymphoma cells

R. E. Meyn, L. C. Stephens, D. W. Voehringer, M. D. Story, N. Mirkovic, L. Milas

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Considerable effort in our laboratory has been directed toward characterizing the role of apoptosis as a mode of cell death in model tumors irradiated in vivo. These studies have shown that apoptosis is an important response in some tumors, correlating with tumor growth delay and tumor cure. However, the response is heterogeneous among both the various tumors examined and the cells in a given tumor, suggesting that the propensity for cells to undergo apoptosis upon irradiation is regulated by unknown factors in tumors. To develop a model system for investigating these regulatory pathways in vitro at the molecular and biochemical levels, we have established cells from a tumor that displays a dramatic apoptotic response in vivo, the TH lymphoma, in cell culture. In this article, we review some of the results of our studies using this model system. To date, we have shown that the dose- response relationship and kinetics of the development of apoptosis for these cells in culture are similar to what we observed for the tumor response in vivo. Moreover, the roles of calcium and signal transduction pathways as important regulatory factors in radiation-induced apoptosis have been defined in this system. Ultimately such investigations may yield the insight necessary for designing protocols to modulate apoptosis biochemically in irradiated normal and tumor tissues to therapeutic advantage.

Original languageEnglish (US)
Pages (from-to)327-334
Number of pages8
JournalRadiation Research
Volume136
Issue number3
StatePublished - 1993

Fingerprint

apoptosis
lymphoma
Lymphoma
tumors
Radiation
Apoptosis
modulation
neoplasms
mice
radiation
cells
Neoplasms
cell culture
Cell Culture Techniques
death
dose response
signal transduction
cell death
calcium
Signal Transduction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Meyn, R. E., Stephens, L. C., Voehringer, D. W., Story, M. D., Mirkovic, N., & Milas, L. (1993). Biochemical modulation of radiation-induced apoptosis in murine lymphoma cells. Radiation Research, 136(3), 327-334.

Biochemical modulation of radiation-induced apoptosis in murine lymphoma cells. / Meyn, R. E.; Stephens, L. C.; Voehringer, D. W.; Story, M. D.; Mirkovic, N.; Milas, L.

In: Radiation Research, Vol. 136, No. 3, 1993, p. 327-334.

Research output: Contribution to journalArticle

Meyn, RE, Stephens, LC, Voehringer, DW, Story, MD, Mirkovic, N & Milas, L 1993, 'Biochemical modulation of radiation-induced apoptosis in murine lymphoma cells', Radiation Research, vol. 136, no. 3, pp. 327-334.
Meyn RE, Stephens LC, Voehringer DW, Story MD, Mirkovic N, Milas L. Biochemical modulation of radiation-induced apoptosis in murine lymphoma cells. Radiation Research. 1993;136(3):327-334.
Meyn, R. E. ; Stephens, L. C. ; Voehringer, D. W. ; Story, M. D. ; Mirkovic, N. ; Milas, L. / Biochemical modulation of radiation-induced apoptosis in murine lymphoma cells. In: Radiation Research. 1993 ; Vol. 136, No. 3. pp. 327-334.
@article{a4330594bfe6438ab73fa30b40273db5,
title = "Biochemical modulation of radiation-induced apoptosis in murine lymphoma cells",
abstract = "Considerable effort in our laboratory has been directed toward characterizing the role of apoptosis as a mode of cell death in model tumors irradiated in vivo. These studies have shown that apoptosis is an important response in some tumors, correlating with tumor growth delay and tumor cure. However, the response is heterogeneous among both the various tumors examined and the cells in a given tumor, suggesting that the propensity for cells to undergo apoptosis upon irradiation is regulated by unknown factors in tumors. To develop a model system for investigating these regulatory pathways in vitro at the molecular and biochemical levels, we have established cells from a tumor that displays a dramatic apoptotic response in vivo, the TH lymphoma, in cell culture. In this article, we review some of the results of our studies using this model system. To date, we have shown that the dose- response relationship and kinetics of the development of apoptosis for these cells in culture are similar to what we observed for the tumor response in vivo. Moreover, the roles of calcium and signal transduction pathways as important regulatory factors in radiation-induced apoptosis have been defined in this system. Ultimately such investigations may yield the insight necessary for designing protocols to modulate apoptosis biochemically in irradiated normal and tumor tissues to therapeutic advantage.",
author = "Meyn, {R. E.} and Stephens, {L. C.} and Voehringer, {D. W.} and Story, {M. D.} and N. Mirkovic and L. Milas",
year = "1993",
language = "English (US)",
volume = "136",
pages = "327--334",
journal = "Radiation Research",
issn = "0033-7587",
publisher = "Radiation Research Society",
number = "3",

}

TY - JOUR

T1 - Biochemical modulation of radiation-induced apoptosis in murine lymphoma cells

AU - Meyn, R. E.

AU - Stephens, L. C.

AU - Voehringer, D. W.

AU - Story, M. D.

AU - Mirkovic, N.

AU - Milas, L.

PY - 1993

Y1 - 1993

N2 - Considerable effort in our laboratory has been directed toward characterizing the role of apoptosis as a mode of cell death in model tumors irradiated in vivo. These studies have shown that apoptosis is an important response in some tumors, correlating with tumor growth delay and tumor cure. However, the response is heterogeneous among both the various tumors examined and the cells in a given tumor, suggesting that the propensity for cells to undergo apoptosis upon irradiation is regulated by unknown factors in tumors. To develop a model system for investigating these regulatory pathways in vitro at the molecular and biochemical levels, we have established cells from a tumor that displays a dramatic apoptotic response in vivo, the TH lymphoma, in cell culture. In this article, we review some of the results of our studies using this model system. To date, we have shown that the dose- response relationship and kinetics of the development of apoptosis for these cells in culture are similar to what we observed for the tumor response in vivo. Moreover, the roles of calcium and signal transduction pathways as important regulatory factors in radiation-induced apoptosis have been defined in this system. Ultimately such investigations may yield the insight necessary for designing protocols to modulate apoptosis biochemically in irradiated normal and tumor tissues to therapeutic advantage.

AB - Considerable effort in our laboratory has been directed toward characterizing the role of apoptosis as a mode of cell death in model tumors irradiated in vivo. These studies have shown that apoptosis is an important response in some tumors, correlating with tumor growth delay and tumor cure. However, the response is heterogeneous among both the various tumors examined and the cells in a given tumor, suggesting that the propensity for cells to undergo apoptosis upon irradiation is regulated by unknown factors in tumors. To develop a model system for investigating these regulatory pathways in vitro at the molecular and biochemical levels, we have established cells from a tumor that displays a dramatic apoptotic response in vivo, the TH lymphoma, in cell culture. In this article, we review some of the results of our studies using this model system. To date, we have shown that the dose- response relationship and kinetics of the development of apoptosis for these cells in culture are similar to what we observed for the tumor response in vivo. Moreover, the roles of calcium and signal transduction pathways as important regulatory factors in radiation-induced apoptosis have been defined in this system. Ultimately such investigations may yield the insight necessary for designing protocols to modulate apoptosis biochemically in irradiated normal and tumor tissues to therapeutic advantage.

UR - http://www.scopus.com/inward/record.url?scp=0027761455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027761455&partnerID=8YFLogxK

M3 - Article

C2 - 8278573

AN - SCOPUS:0027761455

VL - 136

SP - 327

EP - 334

JO - Radiation Research

JF - Radiation Research

SN - 0033-7587

IS - 3

ER -