Biochemical properties and inhibitors of (N-)WASP

Daisy W. Leung, David M. Morgan, Michael K. Rosen

Research output: Contribution to journalReview article

13 Scopus citations

Abstract

The Wiskott-Aldrich syndrome protein (WASP) is an effector of the Rho GTPase Cdc42 and a key component of signaling pathways that regulate the actin cytoskeleton. WASP is regulated by a number of ligands, and the mechanisms by which these act are beginning to be understood through detailed biochemical analyses. Here we describe the protocols we use to study WASP proteins, including the methods we use to purify signaling components and the assays we use to quantitatively characterize the biochemical and biophysical properties of WASP, its activation by Cdc42, and its inhibition by the small molecule wiskostatin. These methods have broad use within the WASP-related cytoskeletal-signaling pathway but are also applicable to investigations of other intramolecular and intermolecular interactions.

Original languageEnglish (US)
Article number21
Pages (from-to)281-296
Number of pages16
JournalMethods in Enzymology
Volume406
DOIs
StatePublished - Jan 1 2006

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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