Biodegradable quantum dot nanocomposites enable live cell labeling and imaging of cytoplasmic targets

Betty Y.S. Kim, Wen Jiang, John Oreopoulos, Christopher M. Yip, James T. Rutka, Warren C.W. Chan

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Semiconductor quantum dots (QDs) offer great promise as the new generation of fluorescent probes to image and study biological processes. Despite their superior optical properties, QDs for live cell monitoring and tracking of cytoplasmic processes remain limited due to inefficient delivery methods available, altered state or function of cells during the delivery process and the requirement of surface-functionalized QDs for specific labeling of subcellular structures. Here, we present a noninvasive method to image subcellular structures in live cells using bioconjugated QD nanocomposites. By incorporating antibody-coated QDs within biodegradable polymeric nanospheres, we have designed a bioresponsive delivery system that undergoes endolysosomal to cytosolic translocation via pH-dependent reversal of nanocomposite surface charge polarity. Upon entering the cytosol, the polymer nanospheres undergo hydrolysis thus releasing the QD bioconjugates. This approach facilitates multiplexed labeling of subcellular structures inside live cells without the requirement of cell fixation or membrane permeabilization. As compared to conventional intracellular delivery techniques, this approach allows the high throughput cytoplasmic delivery of QDs with minimal toxicity to the cell. More importantly, this development demonstrates an important rational strategy for the design of a multifunctional nanosystem for biological applications.

Original languageEnglish (US)
Pages (from-to)3887-3892
Number of pages6
JournalNano Letters
Volume8
Issue number11
DOIs
StatePublished - Nov 1 2008
Externally publishedYes

Fingerprint

Labeling
Semiconductor quantum dots
marking
Nanocomposites
nanocomposites
quantum dots
Imaging techniques
delivery
cells
Nanospheres
Nanosystems
requirements
releasing
Surface charge
antibodies
Fluorescent Dyes
Antibodies
toxicity
Toxicity
hydrolysis

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Biodegradable quantum dot nanocomposites enable live cell labeling and imaging of cytoplasmic targets. / Kim, Betty Y.S.; Jiang, Wen; Oreopoulos, John; Yip, Christopher M.; Rutka, James T.; Chan, Warren C.W.

In: Nano Letters, Vol. 8, No. 11, 01.11.2008, p. 3887-3892.

Research output: Contribution to journalArticle

Kim, Betty Y.S. ; Jiang, Wen ; Oreopoulos, John ; Yip, Christopher M. ; Rutka, James T. ; Chan, Warren C.W. / Biodegradable quantum dot nanocomposites enable live cell labeling and imaging of cytoplasmic targets. In: Nano Letters. 2008 ; Vol. 8, No. 11. pp. 3887-3892.
@article{d6215b1b5bce4ae58c873ab9ba79085a,
title = "Biodegradable quantum dot nanocomposites enable live cell labeling and imaging of cytoplasmic targets",
abstract = "Semiconductor quantum dots (QDs) offer great promise as the new generation of fluorescent probes to image and study biological processes. Despite their superior optical properties, QDs for live cell monitoring and tracking of cytoplasmic processes remain limited due to inefficient delivery methods available, altered state or function of cells during the delivery process and the requirement of surface-functionalized QDs for specific labeling of subcellular structures. Here, we present a noninvasive method to image subcellular structures in live cells using bioconjugated QD nanocomposites. By incorporating antibody-coated QDs within biodegradable polymeric nanospheres, we have designed a bioresponsive delivery system that undergoes endolysosomal to cytosolic translocation via pH-dependent reversal of nanocomposite surface charge polarity. Upon entering the cytosol, the polymer nanospheres undergo hydrolysis thus releasing the QD bioconjugates. This approach facilitates multiplexed labeling of subcellular structures inside live cells without the requirement of cell fixation or membrane permeabilization. As compared to conventional intracellular delivery techniques, this approach allows the high throughput cytoplasmic delivery of QDs with minimal toxicity to the cell. More importantly, this development demonstrates an important rational strategy for the design of a multifunctional nanosystem for biological applications.",
author = "Kim, {Betty Y.S.} and Wen Jiang and John Oreopoulos and Yip, {Christopher M.} and Rutka, {James T.} and Chan, {Warren C.W.}",
year = "2008",
month = "11",
day = "1",
doi = "10.1021/nl802311t",
language = "English (US)",
volume = "8",
pages = "3887--3892",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "11",

}

TY - JOUR

T1 - Biodegradable quantum dot nanocomposites enable live cell labeling and imaging of cytoplasmic targets

AU - Kim, Betty Y.S.

AU - Jiang, Wen

AU - Oreopoulos, John

AU - Yip, Christopher M.

AU - Rutka, James T.

AU - Chan, Warren C.W.

PY - 2008/11/1

Y1 - 2008/11/1

N2 - Semiconductor quantum dots (QDs) offer great promise as the new generation of fluorescent probes to image and study biological processes. Despite their superior optical properties, QDs for live cell monitoring and tracking of cytoplasmic processes remain limited due to inefficient delivery methods available, altered state or function of cells during the delivery process and the requirement of surface-functionalized QDs for specific labeling of subcellular structures. Here, we present a noninvasive method to image subcellular structures in live cells using bioconjugated QD nanocomposites. By incorporating antibody-coated QDs within biodegradable polymeric nanospheres, we have designed a bioresponsive delivery system that undergoes endolysosomal to cytosolic translocation via pH-dependent reversal of nanocomposite surface charge polarity. Upon entering the cytosol, the polymer nanospheres undergo hydrolysis thus releasing the QD bioconjugates. This approach facilitates multiplexed labeling of subcellular structures inside live cells without the requirement of cell fixation or membrane permeabilization. As compared to conventional intracellular delivery techniques, this approach allows the high throughput cytoplasmic delivery of QDs with minimal toxicity to the cell. More importantly, this development demonstrates an important rational strategy for the design of a multifunctional nanosystem for biological applications.

AB - Semiconductor quantum dots (QDs) offer great promise as the new generation of fluorescent probes to image and study biological processes. Despite their superior optical properties, QDs for live cell monitoring and tracking of cytoplasmic processes remain limited due to inefficient delivery methods available, altered state or function of cells during the delivery process and the requirement of surface-functionalized QDs for specific labeling of subcellular structures. Here, we present a noninvasive method to image subcellular structures in live cells using bioconjugated QD nanocomposites. By incorporating antibody-coated QDs within biodegradable polymeric nanospheres, we have designed a bioresponsive delivery system that undergoes endolysosomal to cytosolic translocation via pH-dependent reversal of nanocomposite surface charge polarity. Upon entering the cytosol, the polymer nanospheres undergo hydrolysis thus releasing the QD bioconjugates. This approach facilitates multiplexed labeling of subcellular structures inside live cells without the requirement of cell fixation or membrane permeabilization. As compared to conventional intracellular delivery techniques, this approach allows the high throughput cytoplasmic delivery of QDs with minimal toxicity to the cell. More importantly, this development demonstrates an important rational strategy for the design of a multifunctional nanosystem for biological applications.

UR - http://www.scopus.com/inward/record.url?scp=58149277700&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149277700&partnerID=8YFLogxK

U2 - 10.1021/nl802311t

DO - 10.1021/nl802311t

M3 - Article

C2 - 18816147

AN - SCOPUS:58149277700

VL - 8

SP - 3887

EP - 3892

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 11

ER -