Biodistribution study of nanoparticle encapsulated photodynamic therapy drugs using multispectral imaging

Luma V. Halig, Dongsheng Wang, Andrew Y. Wang, Zhuo Georgia Chen, Baowei Fei

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations

Abstract

Photodynamictherapy (PDT) uses a drug called a photosensitizer that is excited by irradiation with a laser light of a particular wavelength, which generates reactive singlet oxygen that damages the tumor cells. The photosensitizer and light are inert; therefore, systemic toxicities are minimized in PDT. The synthesis of novel PDT drugs and the use of nanosized carriers for photosensitizers may improve the efficiency of the therapy and the delivery of the drug. In this study, we formulated two nanoparticles with and without a targeting ligand to encapsulate phthalocyanines 4 (Pc 4) molecule and compared their biodistributions. Metastatic human head and neck cancer cells (M4e) were transplanted into nude mice. After 2-3 weeks, the mice were injected with Pc 4, Pc 4 encapsulated into surface coated iron oxide (IO-Pc 4), and IO-Pc 4 conjugated with a fibronectin-mimetic peptide (FMP-IO-Pc 4) which binds specifically to integrin β1. The mice were imaged using a multispectral camera. Using multispectral images, a library of spectral signatures was created and the signal per pixel of each tumor was calculated, in a grayscale representation of the unmixed signal of each drug. An enhanced biodistribution of nanoparticle encapsulated PDT drugs compared to non-formulated Pc 4 was observed. Furthermore, specific targeted nanoparticles encapsulated Pc 4 has a quicker delivery time and accumulation in tumor tissue than the non-targeted nanoparticles. The nanoparticle-encapsulated PDT drug can have a variety of potential applications in cancer imaging and treatment.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2013
Subtitle of host publicationBiomedical Applications in Molecular, Structural, and Functional Imaging
DOIs
Publication statusPublished - Jun 3 2013
Externally publishedYes
EventMedical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging - Lake Buena Vista, FL, United States
Duration: Feb 10 2013Feb 13 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8672
ISSN (Print)1605-7422

Conference

ConferenceMedical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging
CountryUnited States
CityLake Buena Vista, FL
Period2/10/132/13/13

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Keywords

  • Drug delivery
  • Drug-encapsulation
  • Head and neck cancer
  • Iron oxide
  • Multispectral imaging
  • Nanomedicine
  • Nanoparticles
  • Optical imaging
  • Pharmaceutical nanocarriers
  • Photodynamic therapy
  • Photosensitizers
  • Phthalocyanine 4 (Pc 4)
  • Theranostic agent

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Halig, L. V., Wang, D., Wang, A. Y., Chen, Z. G., & Fei, B. (2013). Biodistribution study of nanoparticle encapsulated photodynamic therapy drugs using multispectral imaging. In Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging [867218] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8672). https://doi.org/10.1117/12.2006492