@inproceedings{cab9e884809342c9aae5266296298e4c,
title = "Biodistribution study of nanoparticle encapsulated photodynamic therapy drugs using multispectral imaging",
abstract = "Photodynamictherapy (PDT) uses a drug called a photosensitizer that is excited by irradiation with a laser light of a particular wavelength, which generates reactive singlet oxygen that damages the tumor cells. The photosensitizer and light are inert; therefore, systemic toxicities are minimized in PDT. The synthesis of novel PDT drugs and the use of nanosized carriers for photosensitizers may improve the efficiency of the therapy and the delivery of the drug. In this study, we formulated two nanoparticles with and without a targeting ligand to encapsulate phthalocyanines 4 (Pc 4) molecule and compared their biodistributions. Metastatic human head and neck cancer cells (M4e) were transplanted into nude mice. After 2-3 weeks, the mice were injected with Pc 4, Pc 4 encapsulated into surface coated iron oxide (IO-Pc 4), and IO-Pc 4 conjugated with a fibronectin-mimetic peptide (FMP-IO-Pc 4) which binds specifically to integrin β1. The mice were imaged using a multispectral camera. Using multispectral images, a library of spectral signatures was created and the signal per pixel of each tumor was calculated, in a grayscale representation of the unmixed signal of each drug. An enhanced biodistribution of nanoparticle encapsulated PDT drugs compared to non-formulated Pc 4 was observed. Furthermore, specific targeted nanoparticles encapsulated Pc 4 has a quicker delivery time and accumulation in tumor tissue than the non-targeted nanoparticles. The nanoparticle-encapsulated PDT drug can have a variety of potential applications in cancer imaging and treatment.",
keywords = "Drug delivery, Drug-encapsulation, Head and neck cancer, Iron oxide, Multispectral imaging, Nanomedicine, Nanoparticles, Optical imaging, Pharmaceutical nanocarriers, Photodynamic therapy, Photosensitizers, Phthalocyanine 4 (Pc 4), Theranostic agent",
author = "Halig, {Luma V.} and Dongsheng Wang and Wang, {Andrew Y.} and Chen, {Zhuo Georgia} and Baowei Fei",
year = "2013",
doi = "10.1117/12.2006492",
language = "English (US)",
isbn = "9780819494467",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Medical Imaging 2013",
note = "Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging ; Conference date: 10-02-2013 Through 13-02-2013",
}