Biological evaluation of Mycoplasma pulmonis temperature-sensitive mutants for use as possible rodent vaccines

W. C. Lai, M. Bennett, Y. S. Lu, S. P. Pakes

Research output: Contribution to journalArticle

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Abstract

Temperature-sensitive mutants (TSMs) of Mycoplasma pulmonis were produced by treating the wild-type strain with N-methyl-N'-nitro-N-nitrosoguanidine. Three TSms were selected at 38°C, as a restrictive temperature, and at 34°C, as a permissive temperature. Two TSMs, UTCMI and UTCMII, were proven to be nonpathogenic but immunogenic. In addition, they did not induce pneumonia, tracheitis, or tympanitis but did induce mild rhinitis. They were stable after 10 passages in vitro and in vivo. They elicited excellent antibody production and cell-mediated immunity in vaccinated rats. They also were not mitogenic to rat lymphocytes. Rats immunized intranasally with these TSMs were significantly protected against challenge with wild-type organisms. These mutants were morphologically and serologically indistinguishable from the wild-type organisms. The growth characteristics and antibiotic sensitivities were similar to those of wild-type organisms, except that they grew only at 34°C. In contrast to wild-type organisms, they did not bind to or lyse sheep erythrocytes. Thus, these TSMs may qualify as a vaccine to prevent M. pulmonis infection in rats.

Original languageEnglish (US)
Pages (from-to)2289-2296
Number of pages8
JournalInfection and Immunity
Volume58
Issue number7
StatePublished - 1990

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Mycoplasma pulmonis
Rodentia
Vaccines
Temperature
Tracheitis
Mycoplasma Infections
Methylnitronitrosoguanidine
Rhinitis
Cellular Immunity
Antibody Formation
Sheep
Pneumonia
Erythrocytes
Lymphocytes
Anti-Bacterial Agents
Growth

ASJC Scopus subject areas

  • Immunology

Cite this

Biological evaluation of Mycoplasma pulmonis temperature-sensitive mutants for use as possible rodent vaccines. / Lai, W. C.; Bennett, M.; Lu, Y. S.; Pakes, S. P.

In: Infection and Immunity, Vol. 58, No. 7, 1990, p. 2289-2296.

Research output: Contribution to journalArticle

Lai, W. C. ; Bennett, M. ; Lu, Y. S. ; Pakes, S. P. / Biological evaluation of Mycoplasma pulmonis temperature-sensitive mutants for use as possible rodent vaccines. In: Infection and Immunity. 1990 ; Vol. 58, No. 7. pp. 2289-2296.
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