Biological, immunological, and molecular properties of revertants of cat cells transformed by murine sarcoma virus

P. J. Fischinger, C. S. Blevins, A. E. Frankel, N. Tuttle-Fuller, D. K. Haapala, S. Nomura, W. G. Robey

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Abstract

Cloned cat cells (CCC) transformed by the m1 isolate of Moloney murine sarcoma virus (MSV) have the properties of sarcoma-positive, leukemia-negative (S + L -) cells. Over the past 5 years, partially flat as well as very contact-inhibited variant clones were isolated from several clonal generations of S + L - cat cells. Partially flat subclones still contained the MSV genome and could serve as useful cell systems for quantal assays for a number of replicating retroviruses. The frequency of isolation of rescue-negative, very flat subclones diminished with sequential cloning cycles of S + L - cells. The rescue-negative revertant cells grew to low density in liquid media, were more similar to normal cells in soft-agar colony formation, and were intermediate in concanavalin A agglutinability when compared to normal or S + L - cells. Revertants could be retransformed by pseudotypes of MSV other than MSV coated with feline endogenous xenotropic virus. Feline endogenous xenotropic virus was readily induced from S + L - cells but was not inducible from some revertants by halogenated pyrimidines. Rescue-negative revertants could support the growth of a number of helper viruses. Chromosomes of parental CCC normal cells, MSV producer cells, S + L - cells, or revertants were all significantly hypodiploid. No stable pathognomonic changes were observed relative to type and chromosome number among the above. The MSV-coded precursor polyprotein with a molecule weight of 60,000 was detected in producer and S + L - cells but not detected in revertants. Producer and S + L - cells had multiple MSV src copies in their DNA, whereas revertants did not contain residual src DNA. The number of either feline endogenous xenotropic virus or feline leukemia virus-like DNA copies was not different among producer, S + L -, or revertant cells. Accordingly, all rescue-negative cat cell revertants tested have lost multiple copies of MSV proviral DNA.

Original languageEnglish (US)
Pages (from-to)958-965
Number of pages8
JournalCancer Research
Volume41
Issue number3
StatePublished - 1981

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Murine Sarcoma Viruses
Cats
Felidae
DNA
Viruses
Chromosomes
Moloney murine sarcoma virus
Helper Viruses
Feline Leukemia Virus
Polyproteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Fischinger, P. J., Blevins, C. S., Frankel, A. E., Tuttle-Fuller, N., Haapala, D. K., Nomura, S., & Robey, W. G. (1981). Biological, immunological, and molecular properties of revertants of cat cells transformed by murine sarcoma virus. Cancer Research, 41(3), 958-965.

Biological, immunological, and molecular properties of revertants of cat cells transformed by murine sarcoma virus. / Fischinger, P. J.; Blevins, C. S.; Frankel, A. E.; Tuttle-Fuller, N.; Haapala, D. K.; Nomura, S.; Robey, W. G.

In: Cancer Research, Vol. 41, No. 3, 1981, p. 958-965.

Research output: Contribution to journalArticle

Fischinger, PJ, Blevins, CS, Frankel, AE, Tuttle-Fuller, N, Haapala, DK, Nomura, S & Robey, WG 1981, 'Biological, immunological, and molecular properties of revertants of cat cells transformed by murine sarcoma virus', Cancer Research, vol. 41, no. 3, pp. 958-965.
Fischinger PJ, Blevins CS, Frankel AE, Tuttle-Fuller N, Haapala DK, Nomura S et al. Biological, immunological, and molecular properties of revertants of cat cells transformed by murine sarcoma virus. Cancer Research. 1981;41(3):958-965.
Fischinger, P. J. ; Blevins, C. S. ; Frankel, A. E. ; Tuttle-Fuller, N. ; Haapala, D. K. ; Nomura, S. ; Robey, W. G. / Biological, immunological, and molecular properties of revertants of cat cells transformed by murine sarcoma virus. In: Cancer Research. 1981 ; Vol. 41, No. 3. pp. 958-965.
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abstract = "Cloned cat cells (CCC) transformed by the m1 isolate of Moloney murine sarcoma virus (MSV) have the properties of sarcoma-positive, leukemia-negative (S + L -) cells. Over the past 5 years, partially flat as well as very contact-inhibited variant clones were isolated from several clonal generations of S + L - cat cells. Partially flat subclones still contained the MSV genome and could serve as useful cell systems for quantal assays for a number of replicating retroviruses. The frequency of isolation of rescue-negative, very flat subclones diminished with sequential cloning cycles of S + L - cells. The rescue-negative revertant cells grew to low density in liquid media, were more similar to normal cells in soft-agar colony formation, and were intermediate in concanavalin A agglutinability when compared to normal or S + L - cells. Revertants could be retransformed by pseudotypes of MSV other than MSV coated with feline endogenous xenotropic virus. Feline endogenous xenotropic virus was readily induced from S + L - cells but was not inducible from some revertants by halogenated pyrimidines. Rescue-negative revertants could support the growth of a number of helper viruses. Chromosomes of parental CCC normal cells, MSV producer cells, S + L - cells, or revertants were all significantly hypodiploid. No stable pathognomonic changes were observed relative to type and chromosome number among the above. The MSV-coded precursor polyprotein with a molecule weight of 60,000 was detected in producer and S + L - cells but not detected in revertants. Producer and S + L - cells had multiple MSV src copies in their DNA, whereas revertants did not contain residual src DNA. The number of either feline endogenous xenotropic virus or feline leukemia virus-like DNA copies was not different among producer, S + L -, or revertant cells. Accordingly, all rescue-negative cat cell revertants tested have lost multiple copies of MSV proviral DNA.",
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AU - Haapala, D. K.

AU - Nomura, S.

AU - Robey, W. G.

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N2 - Cloned cat cells (CCC) transformed by the m1 isolate of Moloney murine sarcoma virus (MSV) have the properties of sarcoma-positive, leukemia-negative (S + L -) cells. Over the past 5 years, partially flat as well as very contact-inhibited variant clones were isolated from several clonal generations of S + L - cat cells. Partially flat subclones still contained the MSV genome and could serve as useful cell systems for quantal assays for a number of replicating retroviruses. The frequency of isolation of rescue-negative, very flat subclones diminished with sequential cloning cycles of S + L - cells. The rescue-negative revertant cells grew to low density in liquid media, were more similar to normal cells in soft-agar colony formation, and were intermediate in concanavalin A agglutinability when compared to normal or S + L - cells. Revertants could be retransformed by pseudotypes of MSV other than MSV coated with feline endogenous xenotropic virus. Feline endogenous xenotropic virus was readily induced from S + L - cells but was not inducible from some revertants by halogenated pyrimidines. Rescue-negative revertants could support the growth of a number of helper viruses. Chromosomes of parental CCC normal cells, MSV producer cells, S + L - cells, or revertants were all significantly hypodiploid. No stable pathognomonic changes were observed relative to type and chromosome number among the above. The MSV-coded precursor polyprotein with a molecule weight of 60,000 was detected in producer and S + L - cells but not detected in revertants. Producer and S + L - cells had multiple MSV src copies in their DNA, whereas revertants did not contain residual src DNA. The number of either feline endogenous xenotropic virus or feline leukemia virus-like DNA copies was not different among producer, S + L -, or revertant cells. Accordingly, all rescue-negative cat cell revertants tested have lost multiple copies of MSV proviral DNA.

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