Biological roles of filamin a in prostate cancer cells

Xue Chao Li, Chuan Xi Huang, Shi Kui Wu, Lan Yu, Guang Jian Zhou, Li Jun Chen

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: This study aims to investigate the association of filamin A with the function and morphology of prostate cancer (PCa) cells, and explore the role of filamin A in the development of PCa, in order to analyze its significance in the evolvement of PCa. MATERIALS AND METHODS: A stably transfected cell line, in which filamin A expression was suppressed by RNA interference, was first established. Then, the effects of the suppression of filamin A gene expression on the biological characteristics of human PCa LNCaP cells were observed through cell morphology, in vitro cell growth curve, soft agar cloning assay, and scratch test. RESULTS: A cell line model with a low expression of filamin A was successfully constructed on the basis of LNCaP cells. The morphology of cells transfected with plasmid pSilencer-filamin A was the following: Cells were loosely arranged, had less connection with each other, had fewer tentacles, and presented a fibrous look. The growth rate of LNCap cells was faster than cells transfected with plasmid pSilencer-filamin A (P<0.05). The clones of LNCap cells in the soft agar cloning assay was significantly fewer than that of cells stably transfected with plasmid pSilencer-filamin A (P<0.05). Cells stably transfected with plasmid pSilencer-filamin A presented with a stronger healing and migration ability compared to LNCap cells (healing rate was 32.2% and 12.1%, respectively; P<0.05). CONCLUSION: The expression of the filamin A gene inhibited the malignant development of LNCap cells. Therefore, the filamin A gene may be a tumor suppressor gene.

Original languageEnglish (US)
Pages (from-to)916-924
Number of pages9
JournalInternational braz j urol : official journal of the Brazilian Society of Urology
Volume45
Issue number5
DOIs
StatePublished - Sep 1 2019
Externally publishedYes

Keywords

  • Filamins
  • Prostatic Neoplasms
  • RNAi Therapeutics

ASJC Scopus subject areas

  • Urology

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