Biological variability in the structures of diphosphoinositol polyphosphates in Dictyostelium discoideum and mammalian cells

Claudia Albert, Stephen T. Safrany, Michael E. Bembenek, Komandla Malla Reddy, K. Kishta Reddy, J R Falck, Martina Bröcker, Stephen B. Shears, Georg W. Mayr

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


Previous structural analyses of diphosphoinositol polyphosphates in biological systems have relied largely on NMR analysis. For example, in Dictyostelium discoideum, diphosphoinositol pentakisphosphate was determined by NMR to be 4- and/or 6-PPInsP5, and the bisdiphosphoinositol tetrakisphosphate was found to be 4,5-bisPPInsP4 and/or 5,6-bisPPInsP4. We now describe three recent technical developments to aid the analysis of these compounds, not just in Dictyostelium, but also in a wider range of biological systems: (i) improved resolution and sensitivity of detection of PPInsP5 isomers by microbore metal-dye-detection HPLC; (ii) the use of the enantiomerically specific properties of a rat hepatic diphosphatase; (iii) chemical synthesis of enantiomerically pure reference standards of all six possible PPInsP5 isomers. Thus we now demonstrate that the major PPInsP5 isomer in Dictyostelium is 6-PPInsP5. Similar findings obtained using the same synthetic standards have been published. In addition, we show that 10-25% of the Dictyostelium PPInsP5 pool is comprised of 5-PPInsP5. The biological significance of this new observation was reinforced by our demonstration that 5-PPInsP5 is the predominant PPInsP5 isomer in four different mammalian cell lines (FTC human thyroid cancer cells, Swiss 3T3 fibroblasts, Jurkat T-cells and Chinese hamster ovary cells). The fact that the cellular spectrum of diphosphoinositol polyphosphates varies across phylogenetic boundaries underscores the value of our technological developments for future determinations of the structures of this class of compounds in other systems.

Original languageEnglish (US)
Pages (from-to)553-560
Number of pages8
JournalBiochemical Journal
Issue number2
StatePublished - Oct 15 1997

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Biological variability in the structures of diphosphoinositol polyphosphates in Dictyostelium discoideum and mammalian cells'. Together they form a unique fingerprint.

Cite this