Biomarkers of splenic function in infants with sickle cell anemia: Baseline data from the BABYHUG trial

Zora R. Rogers, Winfred C. Wang, Zhaoyu Luo, Rathi V. Iyer, Eglal Shalaby-Rana, Stephen D. Dertinger, Barry L. Shulkin, John H. Miller, Bea Files, Peter A. Lane, Bruce W. Thompson, Scott T. Miller, Russell E. Ware

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

We evaluated spleen function in 193 children with sickle cell anemia 8 to 18 months of age by 99mTc sulfur-colloid liver-spleen scan and correlated results with clinical and laboratory parameters, including 2 splenic biomarkers: pitted cell counts (PIT) and quantitative Howell-Jolly bodies (HJB) enumerated by flow cytometry. Loss of splenic function began before 12 months of age in 86% of infants in association with lower total or fetal hemoglobin and higher white blood cell or reticulocyte counts, reinforcing the need for early diagnosis and diligent preventive care. PIT and HJB correlated well with each other and liver-spleen scan results. Previously described biomarker threshold values did define patients with abnormal splenic function, but our data suggest that normal spleen function is better predicted by PIT of ≤ 1.2% or HJB ≤ 55/106 red blood cells and absent function by PIT ≥ 4.5% or HJB ≥ 665/106. HJB is methodologically advantageous compared with PIT, but both are valid biomarkers of splenic function. This trial was registered at www.clinicaltrials.gov as #NCT00006400.

Original languageEnglish (US)
Pages (from-to)2614-2617
Number of pages4
JournalBlood
Volume117
Issue number9
DOIs
StatePublished - Mar 3 2011

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Rogers, Z. R., Wang, W. C., Luo, Z., Iyer, R. V., Shalaby-Rana, E., Dertinger, S. D., Shulkin, B. L., Miller, J. H., Files, B., Lane, P. A., Thompson, B. W., Miller, S. T., & Ware, R. E. (2011). Biomarkers of splenic function in infants with sickle cell anemia: Baseline data from the BABYHUG trial. Blood, 117(9), 2614-2617. https://doi.org/10.1182/blood-2010-04-278747