Biomechanical and microstructural properties of common carotid arteries from fibulin-5 null mice

William Wan, Hiromi Yanagisawa, Rudolph L. Gleason

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Alteration in the mechanical properties of arteries occurs with aging and disease, and arterial stiffening is a key risk factor for subsequent cardiovascular events. Arterial stiffening is associated with the loss of functional elastic fibers and increased collagen content in the wall of large arteries. Arterial mechanical properties are controlled largely by the turnover and reorganization of key structural proteins and cells, a process termed growth and remodeling. Fibulin-5 (fbln5) is a microfibrillar protein that binds tropoelastin, interacts with integrins, and localizes to elastin fibers; tropoelastin and microfibrillar proteins constitute functional elastic fibers. We performed biaxial mechanical testing and confocal imaging of common carotid arteries (CCAs) from fibulin-5 null mice (fbln5-/-) and littermate controls (fbln5+/+) to characterize the mechanical behavior and microstructural content of these arteries; mechanical testing data were fit to a four-fiber family constitutive model. We found that CCAs from fbln5 -/- mice exhibited lower in vivo axial stretch and lower in vivo stresses while maintaining a similar compliance over physiological pressures compared to littermate controls. Specifically, for fbln5-/- the axial stretch λ = 1.41 ± 0.07, the circumferential stress σθ = 101 ± 32 kPa, and the axial stress σz = 74 ± 28 kPa; for fbln5+/+ λ = 1.64 ± 0.03, σθ = 194 ± 38 kPa, and σz = 159 ± 29 kPa. Structurally, CCAs from fbln5 -/- mice lack distinct functional elastic fibers defined by the lamellar structure of alternating layers of smooth muscle cells and elastin sheets. These data suggest that structural differences in fbln5-/- arteries correlate with significant differences in mechanical properties. Despite these significant differences fbln5-/- CCAs exhibited nearly normal levels of cyclic strain over the cardiac cycle.

Original languageEnglish (US)
Pages (from-to)3605-3617
Number of pages13
JournalAnnals of Biomedical Engineering
Volume38
Issue number12
DOIs
StatePublished - Dec 2010

Fingerprint

Fibers
Elastin
Mechanical testing
Proteins
Mechanical properties
Lamellar structures
Constitutive models
Collagen
Muscle
Aging of materials
Cells
Imaging techniques
Compliance

Keywords

  • Arterial stiffening
  • Extracellular matrix
  • Fibulin-5
  • Mechanobiology
  • Vascular mechanics

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Biomechanical and microstructural properties of common carotid arteries from fibulin-5 null mice. / Wan, William; Yanagisawa, Hiromi; Gleason, Rudolph L.

In: Annals of Biomedical Engineering, Vol. 38, No. 12, 12.2010, p. 3605-3617.

Research output: Contribution to journalArticle

Wan, William ; Yanagisawa, Hiromi ; Gleason, Rudolph L. / Biomechanical and microstructural properties of common carotid arteries from fibulin-5 null mice. In: Annals of Biomedical Engineering. 2010 ; Vol. 38, No. 12. pp. 3605-3617.
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