Biosynthesis of antibiotic 1233A (F-244) and preparation of [14C]1233A

H. Kumagai, H. Tomoda, S. Omura

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The biosynthesis of antibiotic 1233A (F-244) was studied by feeding 13C-labeled precursors to the producing organism, Scopulariopsis sp. F-244. 13C NMR spectroscopy established that 1233A is derived from 4 methionines and 7 acetates. Seven acetates are condensed to form a hexaketide and 4 methyl residues from methionine are introduced into the main skeleton. The β-lactone is derived from the α-carboxylic acid of the hexaketide. Since methionine was efficiently incorporated into 1233A, radiolabeled 1233A was prepared biosynthetically by feeding [14C]methionine to the producer. As a result, [14C]1233A was obtained with high specific radioactivity (27.2μCi/μmol).

Original languageEnglish (US)
Pages (from-to)563-567
Number of pages5
JournalJournal of Antibiotics
Volume45
Issue number4
StatePublished - 1992

Fingerprint

antibiotic 1233A
Methionine
Acetates
Scopulariopsis
Lactones
Carboxylic Acids
Skeleton
Radioactivity
Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Kumagai, H., Tomoda, H., & Omura, S. (1992). Biosynthesis of antibiotic 1233A (F-244) and preparation of [14C]1233A. Journal of Antibiotics, 45(4), 563-567.

Biosynthesis of antibiotic 1233A (F-244) and preparation of [14C]1233A. / Kumagai, H.; Tomoda, H.; Omura, S.

In: Journal of Antibiotics, Vol. 45, No. 4, 1992, p. 563-567.

Research output: Contribution to journalArticle

Kumagai, H, Tomoda, H & Omura, S 1992, 'Biosynthesis of antibiotic 1233A (F-244) and preparation of [14C]1233A', Journal of Antibiotics, vol. 45, no. 4, pp. 563-567.
Kumagai, H. ; Tomoda, H. ; Omura, S. / Biosynthesis of antibiotic 1233A (F-244) and preparation of [14C]1233A. In: Journal of Antibiotics. 1992 ; Vol. 45, No. 4. pp. 563-567.
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