Biosynthesis of N-acetylglucosamine-P-P-dolichol, the committed step of asparagine-linked oligosaccharide assembly

Research output: Contribution to journalArticle

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Abstract

Asparagine-linked glycosylation is initiated by the synthesis of N-acetylglucosaminylpyrophosphoryl dolichol (GlcNAc2-P-P-dolichol), which is extended by a series of glycosyltrans-ferases to yield Glc3Man9GIcNAc2-P-P-dolichol (where Glc is glucose and Man is mannose). The oligosaccharide unit is then transferred en bloc to asparagine residues of nascent polypeptides in the lumen of the rough endoplasmic reticu-lum. The question here is whether GlcNAc-P-P-dolichol biosynthesis is a fixed process unaffected by cellular events, or a regulated reaction responsive to cellular requirements for glycoprotein biosynthesis. Several lines of evidence indicate that the latter is the case and that GlcNAc-P-P-dolichol biosynthesis may be subject to multiple forms of regulation. Recent information about the N-acetylglucosamine-1-P transferase (GPT) responsible for this reaction and the cloning of cDNA candidates for this enzyme have provided further insight into these mechanisms. This review will examine current hypotheses dealing with GPT and its role in the committed step of asparagine-linked glycosylation.

Original languageEnglish (US)
Pages (from-to)553-562
Number of pages10
JournalGlycobiology
Volume1
Issue number6
DOIs
StatePublished - Dec 1991

Fingerprint

Dolichol
Oligosaccharides
Acetylglucosamine
Asparagine
Biosynthesis
Glycosylation
Glycoproteins
Glycoprotein
Cloning
Polypeptides
CDNA
Glucose
Rough
Enzymes
Synthesis
Mannose
Transferases
Unit
Series
Line

Keywords

  • Dolichol
  • Glycosylation
  • N-acetylglucosamine
  • Trans-ferase
  • Tunicamycin

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Hematology
  • Biochemistry

Cite this

Biosynthesis of N-acetylglucosamine-P-P-dolichol, the committed step of asparagine-linked oligosaccharide assembly. / Lehrman, Mark A.

In: Glycobiology, Vol. 1, No. 6, 12.1991, p. 553-562.

Research output: Contribution to journalArticle

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