Biphasic induction of immediate early gene expression accompanies activity-dependent angiogenesis and myofiber remodeling of rabbit skeletal muscle

Jeffrey B. Michel, George A. Ordway, James A. Richardson, R. Sanders Williams

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Sustained contractile activity of skeletal muscle promotes angiogenesis, as well as transformation of contractile protein isoforms and mitochondrial proliferation within myofibers. Since the products of immediate early genes such as c-fos, c-jun, and egr-1 function in many signaling pathways governing cellular responses to external stimuli, we sought to determine whether sustained contractile activity induces their expression in skeletal muscle. Low voltage electrical stimulation was applied to the motor nerve innervating rabbit tibialis anterior muscles for periods ranging from 45 min to 21 d. Northern and Western analysis demonstrated marked but transient inductions of c-fos, c-jun, and egr-1 mRNA and protein within the first 24 h. Longer durations of stimulation were associated with a secondary and sustained rise in the abundance of c-fos, c-jun, and p88(egr-1) protein that, surprisingly, was not accompanied by detectable changes in mRNA. Immunohistochemistry demonstrated c-fos immunoreactivity within myofiber and vascular cell nuclei during both early and late phases of this response. These findings reveal a complex pattern of c-fos, c-jun, and egr-1 expression in response to nerve stimulation and suggest that these proteins could function in regulatory pathways that modify muscle phenotype.

Original languageEnglish (US)
Pages (from-to)277-285
Number of pages9
JournalJournal of Clinical Investigation
Volume94
Issue number1
StatePublished - Jul 1994

Fingerprint

Immediate-Early Genes
Skeletal Muscle
Rabbits
Gene Expression
Contractile Proteins
Muscles
Messenger RNA
Proteins
Cell Nucleus
Electric Stimulation
Blood Vessels
Protein Isoforms
Immunohistochemistry
Phenotype

Keywords

  • c-fos
  • c-jun
  • muscle
  • proto-oncogenes
  • rabbits

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Biphasic induction of immediate early gene expression accompanies activity-dependent angiogenesis and myofiber remodeling of rabbit skeletal muscle. / Michel, Jeffrey B.; Ordway, George A.; Richardson, James A.; Williams, R. Sanders.

In: Journal of Clinical Investigation, Vol. 94, No. 1, 07.1994, p. 277-285.

Research output: Contribution to journalArticle

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