Block of alkaline phosphatase processing due to single amino acid substitutions affects phospholipid content and turnover in E. coli cells secreting the mutant proteins

A. E. Kalinin, A. L. Karamyshev, M. A. Nesmeyanova

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Amino acid substitutions in the signal peptide cleavage site of E. coli alkaline phosphatase (Val for Ala(-1) or Pro for Arg(+1)) impair the enzyme processing and anchoring of precursors in the cytoplasmic membrane. In cells secreting these mutant proteins the relative content and the rate of turnover of anionic phospholipids (phosphatidylglycerol and cardiolipin) are increased. The increase in the transfer of phosphoglycerol residues from phosphatidylglycerol to periplasmic membrane-derived oligosaccharides or to the model substrate arbutin, performed by phosphoglycerol transferase I, indicates increased content of phosphatidylglycerol on the outer side of the cytoplasmic membrane. These results suggest an interaction of phosphatidylglycerol with the alkaline phosphatase precursor followed by their joint translocation across the cytoplasmic membrane of E. coli.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalBiochemistry (Moscow)
Volume61
Issue number1
StatePublished - 1996

Fingerprint

Phosphatidylglycerols
Mutant Proteins
Amino Acid Substitution
Escherichia coli
Alkaline Phosphatase
Phospholipids
Substitution reactions
Membranes
Amino Acids
Cell Membrane
Processing
Arbutin
Cardiolipins
Protein Sorting Signals
Oligosaccharides
Joints
Substrates
Enzymes

Keywords

  • Amino acid substitutions
  • E. coli.
  • PhoA
  • Phospholipids
  • Processing
  • Protein translocation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Block of alkaline phosphatase processing due to single amino acid substitutions affects phospholipid content and turnover in E. coli cells secreting the mutant proteins. / Kalinin, A. E.; Karamyshev, A. L.; Nesmeyanova, M. A.

In: Biochemistry (Moscow), Vol. 61, No. 1, 1996, p. 73-80.

Research output: Contribution to journalArticle

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