TY - JOUR
T1 - Blood-sample processing for the study of age-dependent gene expression in peripheral blood mononuclear cells
AU - Lacelle, Chantale
AU - Riol, Hélène
AU - Xu, Suying
AU - Tang, Yih Jing
AU - Wang, Yuh Shyun
AU - Chuang, Yi Li
AU - Lin, Hui Sheng
AU - Chang, Ming Cheng
AU - Liang, Jersey
AU - Wang, Eugenia
PY - 2002
Y1 - 2002
N2 - Although most new biogerontological studies seeking to identify longevity candidate genes and factors involved in successful human aging are population based, and likely to involve the collection of blood from extremely old individuals, to our knowledge no unified protocols have yet been published to describe a methodology permitting the simultaneous generation of different kinds of biological specimens derived from a single source of a very small volume of peripheral blood. Here we describe a method permitting the simultaneous generation of plasma, RNA, DNA, protein, fixed lymphocytes, and frozen blood aliquots from a single 10- to 30-ml blood sample obtained from donors of any age (10-102 years old), and we show that the quality and quantity of DNA, RNA, protein, and fixed lymphocytes obtained do not vary significantly with age. As is frequently observed, the older individuals have higher plasma proportions.
AB - Although most new biogerontological studies seeking to identify longevity candidate genes and factors involved in successful human aging are population based, and likely to involve the collection of blood from extremely old individuals, to our knowledge no unified protocols have yet been published to describe a methodology permitting the simultaneous generation of different kinds of biological specimens derived from a single source of a very small volume of peripheral blood. Here we describe a method permitting the simultaneous generation of plasma, RNA, DNA, protein, fixed lymphocytes, and frozen blood aliquots from a single 10- to 30-ml blood sample obtained from donors of any age (10-102 years old), and we show that the quality and quantity of DNA, RNA, protein, and fixed lymphocytes obtained do not vary significantly with age. As is frequently observed, the older individuals have higher plasma proportions.
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U2 - 10.1093/gerona/57.7.B285
DO - 10.1093/gerona/57.7.B285
M3 - Article
C2 - 12084799
AN - SCOPUS:18444410738
SN - 1079-5006
VL - 57
SP - B285-B287
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
IS - 7
ER -