TY - JOUR
T1 - BMP and BMP receptor expression during murine organogenesis
AU - Danesh, Shahab M.
AU - Villasenor, Alethia
AU - Chong, Diana
AU - Soukup, Carrie
AU - Cleaver, Ondine
PY - 2009/6
Y1 - 2009/6
N2 - Cell-cell communication is critical for regulating embryonic organ growth and differentiation. The Bone Morphogenetic Protein (BMP) family of transforming growth factor β (TGFβ) molecules represents one class of such cell-cell signaling molecules that regulate the morphogenesis of several organs. Due to high redundancy between the myriad BMP ligands and receptors in certain tissues, it has been challenging to address the role of BMP signaling using targeting of single Bmp genes in mouse models. Here, we present a detailed study of the developmental expression profiles of three BMP ligands (Bmp2, Bmp4, Bmp7) and three BMP receptors (Bmpr1a, Bmpr1b, and BmprII), as well as their molecular antagonist (noggin), in the early embryo during the initial steps of murine organogenesis. In particular, we focus on the expression of Bmp family members in the first organs and tissues that take shape during embryogenesis, such as the heart, vascular system, lungs, liver, stomach, nervous system, somites and limbs. Using in situ hybridization, we identify domains where ligand(s) and receptor(s) are either singly or co-expressed in specific tissues. In addition, we identify a previously unnoticed asymmetric expression of Bmp4 in the gut mesogastrium, which initiates just prior to gut turning and the establishment of organ asymmetry in the gastrointestinal tract. Our studies will aid in the future design and/or interpretation of targeted deletion of individual Bmp or Bmpr genes, since this study identifies organs and tissues where redundant BMP signaling pathways are likely to occur.
AB - Cell-cell communication is critical for regulating embryonic organ growth and differentiation. The Bone Morphogenetic Protein (BMP) family of transforming growth factor β (TGFβ) molecules represents one class of such cell-cell signaling molecules that regulate the morphogenesis of several organs. Due to high redundancy between the myriad BMP ligands and receptors in certain tissues, it has been challenging to address the role of BMP signaling using targeting of single Bmp genes in mouse models. Here, we present a detailed study of the developmental expression profiles of three BMP ligands (Bmp2, Bmp4, Bmp7) and three BMP receptors (Bmpr1a, Bmpr1b, and BmprII), as well as their molecular antagonist (noggin), in the early embryo during the initial steps of murine organogenesis. In particular, we focus on the expression of Bmp family members in the first organs and tissues that take shape during embryogenesis, such as the heart, vascular system, lungs, liver, stomach, nervous system, somites and limbs. Using in situ hybridization, we identify domains where ligand(s) and receptor(s) are either singly or co-expressed in specific tissues. In addition, we identify a previously unnoticed asymmetric expression of Bmp4 in the gut mesogastrium, which initiates just prior to gut turning and the establishment of organ asymmetry in the gastrointestinal tract. Our studies will aid in the future design and/or interpretation of targeted deletion of individual Bmp or Bmpr genes, since this study identifies organs and tissues where redundant BMP signaling pathways are likely to occur.
KW - Bmp2
KW - Bmp4
KW - Bmp7
KW - Bmpr1a
KW - Bmpr1b
KW - BmprII
KW - Gut
KW - Heart
KW - Limb bud
KW - Lung
KW - Neural fold
KW - Noggin
KW - Organogenesis
KW - Somite
KW - Stomach
UR - http://www.scopus.com/inward/record.url?scp=67349109704&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67349109704&partnerID=8YFLogxK
U2 - 10.1016/j.gep.2009.04.002
DO - 10.1016/j.gep.2009.04.002
M3 - Article
C2 - 19393343
AN - SCOPUS:67349109704
SN - 1567-133X
VL - 9
SP - 255
EP - 265
JO - Gene Expression Patterns
JF - Gene Expression Patterns
IS - 5
ER -