Abstract
The bone morphogenetic protein (BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to define functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development.
Original language | English (US) |
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Pages (from-to) | 2177-2187 |
Number of pages | 11 |
Journal | Development |
Volume | 133 |
Issue number | 11 |
DOIs | |
State | Published - Jun 2006 |
Keywords
- Endoythelial
- Hematopoiesis
- Imo2
- Pronephros
- Transgenic
- Zebrafish
ASJC Scopus subject areas
- Molecular Biology
- Developmental Biology