BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis

Sunny Gupta, Hao Zhu, Leonard I. Zon, Todd Evans

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The bone morphogenetic protein (BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to define functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development.

Original languageEnglish (US)
Pages (from-to)2177-2187
Number of pages11
JournalDevelopment
Volume133
Issue number11
DOIs
StatePublished - Jun 1 2006

Keywords

  • Endoythelial
  • Hematopoiesis
  • Imo2
  • Pronephros
  • Transgenic
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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