Bmp15 Is an Oocyte-Produced Signal Required for Maintenance of the Adult Female Sexual Phenotype in Zebrafish

Daniel B. Dranow, Kevin Hu, April M. Bird, S. Terese Lawry, Melissa T. Adams, Angelica Sanchez, James F. Amatruda, Bruce W. Draper

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Although the zebrafish is a major model organism, how they determine sex is not well understood. In domesticated zebrafish, sex determination appears to be polygenic, being influenced by multiple genetic factors that may vary from strain to strain, and additionally can be influenced by environmental factors. However, the requirement of germ cells for female sex determination is well documented: animals that lack germ cells, or oocytes in particular, develop exclusively as males. Recently, it has been determined that oocytes are also required throughout the adult life of the animal to maintain the differentiated female state. How oocytes control sex differentiation and maintenance of the sexual phenotype is unknown. We therefore generated targeted mutations in genes for two oocyte produced signaling molecules, Bmp15 and Gdf9 and here report a novel role for Bmp15 in maintaining adult female sex differentiation in zebrafish. Females deficient in Bmp15 begin development normally but switch sex during the mid- to late- juvenile stage, and become fertile males. Additionally, by generating mutations in the aromatase cyp19a1a, we show that estrogen production is necessary for female development and that the function of Bmp15 in female sex maintenance is likely linked to the regulation of estrogen biosynthesis via promoting the development of estrogen-producing granulosa cells in the oocyte follicle.

Original languageEnglish (US)
Article numbere1006323
JournalPLoS Genetics
Volume12
Issue number9
DOIs
StatePublished - Sep 1 2016

Fingerprint

Zebrafish
Danio rerio
Oocytes
phenotype
oocytes
Maintenance
Phenotype
gender
estrogens
Sex Differentiation
Estrogens
sex determination
germ cell
sexual development
Germ Cells
mutation
germ cells
Mutation
Aromatase
Granulosa Cells

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Dranow, D. B., Hu, K., Bird, A. M., Lawry, S. T., Adams, M. T., Sanchez, A., ... Draper, B. W. (2016). Bmp15 Is an Oocyte-Produced Signal Required for Maintenance of the Adult Female Sexual Phenotype in Zebrafish. PLoS Genetics, 12(9), [e1006323]. https://doi.org/10.1371/journal.pgen.1006323

Bmp15 Is an Oocyte-Produced Signal Required for Maintenance of the Adult Female Sexual Phenotype in Zebrafish. / Dranow, Daniel B.; Hu, Kevin; Bird, April M.; Lawry, S. Terese; Adams, Melissa T.; Sanchez, Angelica; Amatruda, James F.; Draper, Bruce W.

In: PLoS Genetics, Vol. 12, No. 9, e1006323, 01.09.2016.

Research output: Contribution to journalArticle

Dranow, DB, Hu, K, Bird, AM, Lawry, ST, Adams, MT, Sanchez, A, Amatruda, JF & Draper, BW 2016, 'Bmp15 Is an Oocyte-Produced Signal Required for Maintenance of the Adult Female Sexual Phenotype in Zebrafish', PLoS Genetics, vol. 12, no. 9, e1006323. https://doi.org/10.1371/journal.pgen.1006323
Dranow, Daniel B. ; Hu, Kevin ; Bird, April M. ; Lawry, S. Terese ; Adams, Melissa T. ; Sanchez, Angelica ; Amatruda, James F. ; Draper, Bruce W. / Bmp15 Is an Oocyte-Produced Signal Required for Maintenance of the Adult Female Sexual Phenotype in Zebrafish. In: PLoS Genetics. 2016 ; Vol. 12, No. 9.
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