Bone marrow cell transplants involving donors and hosts with haplotypes derived from spretus mice

M. Bennett, T. D'Orazio, V. Kumar, D. Stenoien, K. C. Blomer, K. F. Lindahl

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Intra-H2 recombinant inbred mice derived from matings between B10 (H2b) and B10.SP2 (H2(sp2)) mice, with an H2 haplotype derived from Mus spretus, have been used to map genes at H2. Recombinants 10115, 10484, R40, 9347, and 9950 were used as donors or hosts in bone marrow cell (BMC) transplants in irradiated mice. From previous studies of Mus musculus mice, the antigens (Ag) on BMC appear to be inherited recessively. The mechanisms offered include codominant inheritance of transacting genes that regulate expression of BMC Ag (Hh hypothesis) and codominant inheritance of class I Ag motifs capable of sending 'negative signals' to effector natural killer (NK) cells (missing serf hypothesis). Our results indicate that stem cell donors that express the same class I Ag, but differ at genes between Bat2 and Tnfa in the H2-S/D interval, can differ in immunogenicity of transplanted stem cells. The structural gene for the H2(sp2) Ag appears to map telomeric of Bat2 and is codominantly inherited. An H2b gene capable of inhibiting expression of the H2(sp2) Ag (or contributing to class I motifs capable of inhibiting NK cell mediated lysis of H2(sp2) BMC) maps in the Bat2f/Tnfa gene segment, but requires homozygosity for this function and may require the H2-Db gene as well. Although H2(sp2) hosts reject H2b BMC, hosts (10115, 10484, R40, and 9347 strain) that are H2b in the centromeric, and H2(sp2) in the telomeric, portion of H2 accept H2b BMC grafts. These two observations have not been made with haplotypes entirely of Mus musculus origin. The data do not support the Hh hypothesis, and are consistent with the missing self hypothesis only if the gene (requiring homozygozity for function) in the Bat2/Tnfa region codes for a particular protein or peptide that associates with Db to generate a 'protective motif.'

Original languageEnglish (US)
Pages (from-to)1452-1459
Number of pages8
JournalTransplantation
Volume59
Issue number10
StatePublished - 1995

Fingerprint

Bone Marrow Cells
Haplotypes
Transplants
Allura Red AC Dye
Genes
H-2 Antigens
Histocompatibility Antigens Class I
Natural Killer Cells
Stem Cells
Antigens
Gene Expression
Peptides
Proteins

ASJC Scopus subject areas

  • Immunology
  • Transplantation

Cite this

Bennett, M., D'Orazio, T., Kumar, V., Stenoien, D., Blomer, K. C., & Lindahl, K. F. (1995). Bone marrow cell transplants involving donors and hosts with haplotypes derived from spretus mice. Transplantation, 59(10), 1452-1459.

Bone marrow cell transplants involving donors and hosts with haplotypes derived from spretus mice. / Bennett, M.; D'Orazio, T.; Kumar, V.; Stenoien, D.; Blomer, K. C.; Lindahl, K. F.

In: Transplantation, Vol. 59, No. 10, 1995, p. 1452-1459.

Research output: Contribution to journalArticle

Bennett, M, D'Orazio, T, Kumar, V, Stenoien, D, Blomer, KC & Lindahl, KF 1995, 'Bone marrow cell transplants involving donors and hosts with haplotypes derived from spretus mice', Transplantation, vol. 59, no. 10, pp. 1452-1459.
Bennett M, D'Orazio T, Kumar V, Stenoien D, Blomer KC, Lindahl KF. Bone marrow cell transplants involving donors and hosts with haplotypes derived from spretus mice. Transplantation. 1995;59(10):1452-1459.
Bennett, M. ; D'Orazio, T. ; Kumar, V. ; Stenoien, D. ; Blomer, K. C. ; Lindahl, K. F. / Bone marrow cell transplants involving donors and hosts with haplotypes derived from spretus mice. In: Transplantation. 1995 ; Vol. 59, No. 10. pp. 1452-1459.
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AU - Bennett, M.

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AU - Lindahl, K. F.

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