Bone marrow T cells are superior to splenic T cells to induce chimeric conversion after non-myeloablative bone marrow transplantation

Hyun Sil Park, Seok Goo Cho, Min Jung Park, So Youn Min, Hong Seok Chang, Hee Je Kim, Seok Lee, Chang Ki Min, Jong Wook Lee, Woo Sung Min, Chun Choo Kim, Ho Youn Kim

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Background/Aims: The bone marrow functions not only as the primary B-lymphocyte-producing organ but also as a secondary lymphoid organ for CD4 and CD8 cell responses and a site of preferential homing and persistence for memory T cells. Bone marrow T (BM-T) cells are distinguished from peripheral blood T cells by surface phenotype, cytokine secretion profile, and immune functions. In this study, we evaluated the alloreactive potential of donor lymphocyte infusion (DLI) using BM-T cells in mixed chimerism compared to that using spleen T (SP-T) cells. Methods: Cells were prepared using established procedures. BM-T cells were obtained as a by-product of T-cell depletion in BM grafting and then cryopreserved for subsequent DLI. We performed DLI using BM-T cells in allogeneic mixed chimera mice on post-BMT day 21. Results: When the same dose of T cells, 5-10×105 (Thy1.2+), fractionated from BM and spleen were administered into mixed chimeras, the BM-T group showed complete chimeric conversion, with self-limited graft-versus-host disease (GVHD) and no pathological changes. However, the SP-T group showed persistent mixed chimerism, with pathological signs of GVHD in the liver and intestine. Conclusions: Our results suggest that DLI using BM-T cells, even in small numbers, is more potent at inducing chimeric conversion in mixed chimerism than DLI using SP-T cells. Further study is needed to determine whether cryopreserved BM-T cells are an effective cell source for DLI to consolidate donor-dominant chimerism in clinical practice without concerns about GVHD.

Original languageEnglish (US)
Pages (from-to)252-262
Number of pages11
JournalKorean Journal of Internal Medicine
Volume24
Issue number3
DOIs
Publication statusPublished - 2009

    Fingerprint

Keywords

  • Chimerism, mixed
  • Infusion, donor lymphocyte
  • T-cells, bone marrow

ASJC Scopus subject areas

  • Internal Medicine

Cite this