Bovine heart fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase: Complete amino acid sequence and localization of phosphorylation sites

Junichiro Sakata, Kosaku Uyeda

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

We have shown previously that bovine heart fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase (EC 2.7.1.105/3.1.3.46) is phosphorylated by cAMP-dependent protein kinase and protein kinase C; phosphorylation results in activation of kinase. This activation of heart enzyme is in contrast to results with the liver isozyme, in which phosphorylation by cAMP-dependent protein kinase inhibits the kinase activity. As an initial step toward understanding this difference between the isozymes we have determined the DNA sequence of the heart enzyme and analyzed the amino acid sequence with special emphasis on the location of the phosphorylation site. We isolated and sequenced two overlapping cDNA fragments, which together could encode the complete amino acid sequence of bovine heart fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, a protein of 530 amino acids, with a calculated molecular weight of 60,679. Since the deduced protein contained amino acid sequences identical to the sequences of four known tryptic peptides from this enzyme we concluded that the deduced protein sequence did represent bovine heart enzyme. In addition, a cDNA fragment hybridized to a 4-kilobase mRNA from bovine heart. The phosphorylation sites of the heart enzyme were located near the C terminus, whereas the phosphorylation site of the liver isozyme is known to be located near the N terminus. These opposite locations of the phosphorylation sites may explain the contrasting effect of the covalent modification on the enzymes' activities.

Original languageEnglish (US)
Pages (from-to)4951-4955
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number13
DOIs
StatePublished - Jan 1 1990

Keywords

  • Fructose 2,6-bisphosphate
  • Glycolysis

ASJC Scopus subject areas

  • General

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