Bovine steroid 21-hydroxylase

Regulation of biosynthesis

Maliyakal E. John, Takashi Okamura, Albert Dee, Beverly Adler, Manorama C. John, Perrin C. White, Evan R. Simpson, Michael R. Waterman

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

A recombinant cDNA clone, PBC21-1, specific for bovine steroid 21-hydroxylase cytochrome P-450 (P-450C21) was identified in a bovine adrenocortical cDNA library, and this identity was confirmed by nucleotide sequencing which revealed significant amino acid homology (77%) with human P-450C21 cDNA. The pBC21-1 insert is 1.7 kilobases in length and includes a 1128 base pair region that encodes the C-terminal 376 amino acids of bovine P-450C21 as well as 535 base pairs of 3′-untranslated sequence. A novel feature of this insert is a 20 base pair intervening sequence near the 5′ end, apparently the result of an aberrant splicing event. Northern blot analysis reveals that bovine P-450C21 is encoded by two transcripts, 2.3 and 2.0 kilobases in length which are detected in adrenal cortical RNA. Bovine liver, heart, kidney, and corpus luteum do not contain detectable P-450C21 transcripts. Regulation of P-450C21 gene expression by adrenocorticotropin was investigated with pBC21-1 and bovine adrenocortical cells in primary, monolayer culture. Treatment with ACTH or analogues of cAMP increases the steady-state levels of P-450C21 RNA in such cell cultures. In vitro transcription run-on assays suggest that this increase is, at least in part, due to the enhanced transcriptional activity of the P-450C21 gene.

Original languageEnglish (US)
Pages (from-to)2846-2853
Number of pages8
JournalBiochemistry
Volume25
Issue number10
StatePublished - 1986

Fingerprint

Steroid 21-Hydroxylase
Biosynthesis
Adrenocorticotropic Hormone
Complementary DNA
RNA
Amino Acids
Transcription
Gene Library
Cell culture
Gene expression
Base Pairing
Liver
Introns
Cytochrome P-450 Enzyme System
Monolayers
Assays
Nucleotides
Genes
Corpus Luteum
Northern Blotting

ASJC Scopus subject areas

  • Biochemistry

Cite this

John, M. E., Okamura, T., Dee, A., Adler, B., John, M. C., White, P. C., ... Waterman, M. R. (1986). Bovine steroid 21-hydroxylase: Regulation of biosynthesis. Biochemistry, 25(10), 2846-2853.

Bovine steroid 21-hydroxylase : Regulation of biosynthesis. / John, Maliyakal E.; Okamura, Takashi; Dee, Albert; Adler, Beverly; John, Manorama C.; White, Perrin C.; Simpson, Evan R.; Waterman, Michael R.

In: Biochemistry, Vol. 25, No. 10, 1986, p. 2846-2853.

Research output: Contribution to journalArticle

John, ME, Okamura, T, Dee, A, Adler, B, John, MC, White, PC, Simpson, ER & Waterman, MR 1986, 'Bovine steroid 21-hydroxylase: Regulation of biosynthesis', Biochemistry, vol. 25, no. 10, pp. 2846-2853.
John ME, Okamura T, Dee A, Adler B, John MC, White PC et al. Bovine steroid 21-hydroxylase: Regulation of biosynthesis. Biochemistry. 1986;25(10):2846-2853.
John, Maliyakal E. ; Okamura, Takashi ; Dee, Albert ; Adler, Beverly ; John, Manorama C. ; White, Perrin C. ; Simpson, Evan R. ; Waterman, Michael R. / Bovine steroid 21-hydroxylase : Regulation of biosynthesis. In: Biochemistry. 1986 ; Vol. 25, No. 10. pp. 2846-2853.
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