TY - JOUR
T1 - Bradykinin modulates potassium and calcium currents in neuroblastoma hybrid cells via different pertussis toxin-insensitive pathways
AU - Wilk-Blaszczak, M. A.
AU - Gutowski, S.
AU - Sternweis, P. C.
AU - Belardetti, F.
N1 - Funding Information:
custom-modified version of the Log-Rank software, A. S. French for the graphic software, P. Middleton, M. A. Harrington, M. L. Burleson, and S. Siegelbaum for comments on the manuscript, and K. Edwards for expert and patient secretarial assistance. This work was supported by USPHS grant GM31954 to P. S., USPHS grants CM47721 and NS25186 to F. B., and a Klingenstein Fellowship to F. 8.; F. B. is an Established Investigator of the American Heart Association.
PY - 1994/1
Y1 - 1994/1
N2 - In NG 108-15 cells, bradykinin (BK) activates a potassium current (IK,BK) and inhibits the voltage-dependent calcium current (ICa,V). BK also stimulates a phosphatidylinositol-specific phospholipase C (PI-PLC). The subsequent release of inositol 1,4,5-trisphosphate and increase in intracellular calcium contribute to IK,BK, through activation of a calcium-dependent potassium current. In membranes from these cells, stimulation of PI-PLC by BK is mediated by Gq and/or G11 two homologous, pertussis toxin-insensitive G proteins. Here, we have investigated the role of Gq/l1 in the electrical responses to BK. GTPγS mimicked and occluded both actions of BK, and both effects were insensitive to pertussis toxin. Perfusion of an anti-Gq/11α antibody into the pipette suppressed IK,BK, but not the inhibition of ICa,V by BK. Thus, BK couples to IK,BK via Gq/11, but coupling to ICa,V is most likely via a different, pertussis toxin-insensitive G protein.
AB - In NG 108-15 cells, bradykinin (BK) activates a potassium current (IK,BK) and inhibits the voltage-dependent calcium current (ICa,V). BK also stimulates a phosphatidylinositol-specific phospholipase C (PI-PLC). The subsequent release of inositol 1,4,5-trisphosphate and increase in intracellular calcium contribute to IK,BK, through activation of a calcium-dependent potassium current. In membranes from these cells, stimulation of PI-PLC by BK is mediated by Gq and/or G11 two homologous, pertussis toxin-insensitive G proteins. Here, we have investigated the role of Gq/l1 in the electrical responses to BK. GTPγS mimicked and occluded both actions of BK, and both effects were insensitive to pertussis toxin. Perfusion of an anti-Gq/11α antibody into the pipette suppressed IK,BK, but not the inhibition of ICa,V by BK. Thus, BK couples to IK,BK via Gq/11, but coupling to ICa,V is most likely via a different, pertussis toxin-insensitive G protein.
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U2 - 10.1016/0896-6273(94)90156-2
DO - 10.1016/0896-6273(94)90156-2
M3 - Article
C2 - 8292355
AN - SCOPUS:0028082134
SN - 0896-6273
VL - 12
SP - 109
EP - 116
JO - Neuron
JF - Neuron
IS - 1
ER -